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Large-Scale Analysis of Gene Expression Profiles during Early Stages of Root Nodule Formation in a Model Legume, Lotus japonicus (2004)
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BibTeX
@MISC{Kouchi04large-scaleanalysis,
author = {Hiroshi Kouchi and Kenshiro Shimomura and Shingo Hata and Atsuko Hirota and Guo-jiang Wu and Daisuke Shibata},
title = {Large-Scale Analysis of Gene Expression Profiles during Early Stages of Root Nodule Formation in a Model Legume, Lotus japonicus},
year = {2004}
}
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Abstract
Gene expression profiles during early stages of formation of symbiotic nitrogen-fixing nodules in a model legume Lotus japonicus were analyzed by means of a cDNA array of 18,144 non-redundant expressed sequence tags (ESTs) isolated from L. japonicus. Expression of a total of 1,076 genes was significantly accelerated during the successive stages that represent infection of Mesorhizobium loti, nodule primordium initiation, nodule organogenesis, and the onset of nitrogen fixation. These include 32 nodulin and nodulinhomolog genes as well as a number of genes involved in the catabolism of photosynthates and assimilation of fixed nitrogen that were previously known to be abundantly expressed in root nodules of many legumes. We also identified a large number of novel nodule-specific or enhanced genes, which include genes involved in many cellular processes such as membrane transport, defense responses, phytohormone synthesis and responses, signal transduction, cell wall synthesis, and transcriptional regulation. Notably, our data indicate that the gene expression profile in early steps of Rhizobium-legume interactions is considerably different from that in subsequent stages of nodule development. A number of genes involved in the defense responses
Keyphrases
gene expression profile early stage lotus japonicus large-scale analysis root nodule formation model legume defense response membrane transport enhanced gene phytohormone synthesis many legume model legume lotus japonicus fixed nitrogen root nodule early step cdna array symbiotic nitrogen-fixing nodule rhizobium-legume interaction nodulinhomolog gene transcriptional regulation many cellular process subsequent stage nitrogen fixation cell wall synthesis mesorhizobium loti nodule organogenesis sequence tag signal transduction nodule primordium initiation large number nodule development successive stage
