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Biotechnology
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@MISC{And_biotechnology,
author = {For Fuels And and Biotechnology and Mark Finkelstein and James D. Mcmillan and Brian H. Davison and Barbara Evans and Melvin P. Tucker and Nicholas J. Nagle and Edward W. Jennings and Kelly N. Ibsen and Andy Aden and Quang A. Nguyen and Kyoung H. Kim and Sally L. Noll},
title = {Biotechnology},
year = {}
}
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Abstract
Over the past three decades ethanol production in the United States has increased more than 10-fold, to approx 2.9 billion gal/yr (mid-2003), with ethanol production expected to reach 5 billion gal/yr by 2005. The simultaneous coproduction of 7 million t/yr of distiller's grain (DG)may potentially drive down the price of DG as a cattle feed supplement. The sale of residual DG for animalfeed is animportant part of corndry-grindethanolproduction economics; therefore, dry-grind ethanol producers are seeking ways to improve the quality of DG to increase market penetration and help stabilize prices. One possible improvement is to increase the protein content of DGby converting the residual starch and fiber into ethanol. We have developed methods for steam explosion, SO 2 , and dilute--sulfuric acid pretreatment of DG for evaluation as a feedstock for ethanol production. The highest soluble sugar yields (~77% of available carbohydrate) were obtained by pretreatment of DG at 140C for 20 min with 3.27 wt% H 2 SO 4 . Fermentation protocols for pretreated DGwere developed at the bench scale and scaled to a working volume of 809 L for production of hydrolyzed distiller's grain (HDG) for feeding trials. ThepretreatedDGwasfermentedwithSaccharomyces cerevisiae D 5 A, with ethanol yields of 73% of theoretical from available glucans.
Keyphrases
ethanol production protein content fermentation protocol possible improvement corndry-grindethanolproduction economics market penetration residual starch steam explosion bench scale cattle feed supplement gal yr animportant part sulfuric acid pretreatment decade ethanol production available glucans dry-grind ethanol producer ethanol yield hydrolyzed distiller residual dg simultaneous coproduction available carbohydrate united state soluble sugar yield working volume
