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Induction of pluripotent stem cells from adult human fibroblasts by defined factors
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| Venue: | Cell 2007 |
| Citations: | 446 - 3 self |
BibTeX
@ARTICLE{Takahashi_inductionof,
author = {Kazutoshi Takahashi and Koji Tanabe and Mari Ohnuki and Megumi Narita and Tomoko Ichisaka and Kiichiro Tomoda},
title = {Induction of pluripotent stem cells from adult human fibroblasts by defined factors},
journal = {Cell 2007},
year = {},
pages = {861--872}
}
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Abstract
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
Keyphrases
adult human fibroblast pluripotent stem cell defined factor ip cell telomerase activity human ip cell germline transmission induced pluripotent stem differentiated human somatic cell disease-specific stem cell gene expression pluripotent state surface antigen mouse somatic cell pluripotent cell-specific gene defined transcription factor germ layer epigenetic status adult human dermal fibroblast successful reprogramming cell type human embryonic stem
