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Alternative splicing regulation at tandem 3 0 splice sites (2006)
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| Venue: | Nucleic Acids Res |
| Citations: | 4 - 1 self |
BibTeX
@ARTICLE{Akerman06alternativesplicing,
author = {Martin Akerman and Yael M},
title = {Alternative splicing regulation at tandem 3 0 splice sites},
journal = {Nucleic Acids Res},
year = {2006},
volume = {34},
pages = {23--31}
}
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Abstract
Alternative splicing (AS) constitutes a major mechanism creating protein diversity in humans. Previous bioinformatics studies based on expressed sequence tag and mRNA data have identified many AS events that are conserved between humans and mice. Of these events, 25 % are related to alternative choices of 30 and 50 splice sites. Surprisingly, half of all these events involve 30 splice sites that are exactly 3 nt apart. These tandem 30 splice sites result from the presence of the NAGNAG motif at the acceptor splice site, recently reported to be widely spread in the human genome. Although the NAGNAG motif is common in human genes, only a small subset of sites with this motif is confirmed to be involved in AS. We examined the NAGNAG motifs and observed specific features such as high sequence conservation of the motif, high conservation of 30 bp at the intronic regions flanking the 30 splice site and overabundance of cis-regulatory elements, which are characteristic of alternatively spliced tandem acceptor sites and can distinguish them from the constitutive sites in which the proximal NAG splice site is selected. Our findings imply that AS at tandem splice sites and constitutive splicing of the distal NAG are highly regulated.
Keyphrases
splice site nagnag motif protein diversity distal nag human gene major mechanism specific feature proximal nag splice site expressed sequence tag previous bioinformatics study alternative choice human genome alternative splicing constitutive site high conservation tandem acceptor site small subset constitutive splicing cis-regulatory element mrna data high sequence conservation tandem splice site intronic region acceptor splice site
