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ONE-STEP GROWTH CURVES OF VARIOUS STRAINS OF IN- FLUENZA A AND B VIRUSES AND THEIR INHIBITION BY INACTIVATED VIRUS OF THE HOMOLOGOUS TYPE*
BibTeX
@MISC{Henle_one-stepgrowth,
author = {Werner Henle and Azcd Evelyn and B. Rosenberg},
title = {ONE-STEP GROWTH CURVES OF VARIOUS STRAINS OF IN- FLUENZA A AND B VIRUSES AND THEIR INHIBITION BY INACTIVATED VIRUS OF THE HOMOLOGOUS TYPE*},
year = {}
}
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Abstract
Studies on the propagation of influenza viruses in the chick embryo (I) have shown that under certain conditions one-step growth curves may be obtained which are similar in principle to those observed in bacterial host-virus systems (2, 3). Upon aUantoic injection of either the PR8 strain of influenza A or the Lee strain of influenza B virus, only part of the active virus is adsorbed by the ceils lining the allantoic cavity. The amount of residual free virus of the seed, which can be assayed in the allantoic fluid of the injected eggs by infectivity titrations, remains constant for a period of 5 to 6 hours in the case of the PR8 strain, and for 8 to 9 hours in that of the Lee strain. After this constant period, the virus titer increases as a result of fiberation of newly formed virus from the infected tissue. Part of the released virus is adsorbed immediately upon some of the remaining uninfected host cells, and the rise in titer in the aUantoic fluid is consequently relatively slow. However, if one induces the interference phenomenon in the remaining susceptible cells by injection of large amounts of heterologous virus inactivated by irradiation with ultraviolet light (4, 5), most
Keyphrases
homologous type lee strain pr8 strain influenza virus residual free virus constant period heterologous virus infectivity titration auantoic injection bacterial host-virus system virus titer increase infected tissue auantoic fluid certain condition one-step growth curve interference phenomenon allantoic cavity susceptible cell chick embryo ultraviolet light allantoic fluid active virus large amount uninfected host cell
