DMCA
Molecular Cell Article Genome-wide Measurement of RNA Folding Energies
BibTeX
@MISC{Wan_molecularcell,
author = {Yue Wan and Kun Qu and Zhengqing Ouyang and Michael Kertesz and Jun Li and Robert Tibshirani and Debora L. Makino and Robert C. Nutter and Eran Segal and Howard Y. Chang and Program In Epithelial Biology},
title = {Molecular Cell Article Genome-wide Measurement of RNA Folding Energies},
year = {}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
RNA structural transitions are important in the function and regulation of RNAs. Here, we reveal a layer of transcriptome organization in the form of RNA folding energies. By probing yeast RNA structures at different temperatures, we obtained relative melting temperatures (Tm) for RNA structures in over 4000 transcripts. Specific signatures of RNA Tm demarcated the polarity of mRNA open reading frames and highlighted numerous candidate regulatory RNA motifs in 3 0 untranslated regions. RNA Tm distinguished noncoding versus coding RNAs and identified mRNAs with distinct cellular functions. We identified thousands of putative RNA thermometers, and their presence is predictive of the pattern of RNA decay in vivo during heat shock. The exosome complex recognizes unpaired bases during heat shock to degrade these RNAs, coupling intrinsic structural stabilities to gene regulation. Thus, genome-wide structural dynamics of RNA can parse functional elements of the transcriptome and reveal diverse biological insights.
Keyphrases
molecular cell article genome-wide measurement rna folding energy rna tm heat shock relative melting temperature rna decay functional element rna structural transition rna structure reveal diverse biological insight gene regulation specific signature numerous candidate regulatory rna motif exosome complex genome-wide structural dynamic unpaired base distinct cellular function yeast rna intrinsic structural stability putative rna thermometer untranslated region different temperature mrna open reading frame transcriptome organization
