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Assessment of Occupational Genotoxic Risk in the Production of Rubber Tyres (2006)
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BibTeX
@MISC{Laffon06assessmentof,
author = {Blanca Laffon and Joao Paulo Teixeira and Susana Silva and Joana Roma-torres and Olga Mayan},
title = {Assessment of Occupational Genotoxic Risk in the Production of Rubber Tyres},
year = {2006}
}
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Abstract
A broad spectrum of substances is used in the rubber industry, many of them being genotoxic and/or carcinogenic. Convincing evidence of an excess of certain forms of cancer among rubber workers has been provided. The objective of this study was to determine the genotoxic effects in a group of individuals engaged in the production of rubber tyres from a Portuguese factory. Peripheral blood samples were collected from 32 exposed workers and 32 controls, and micronucleus (MN) test, sister chromatid exchanges (SCE) and comet assay were performed. Urinary thioethers were measured as a general biomarker of exposure to electrophilic com-pounds, and genetic polymorphisms in metabolizing enzymes (CYP2E1 Dra I, EPHX1 codons 113 and 139, GSTP1 codon 105, and GSTM1 and GSTT1 deletion polymorphisms) were analysed as susceptibility biomarkers. Excretion of thioethers was found significantly higher in rubber workers. Also, a non-significant increase inMN frequency related to time of exposure and no effect in SCE were observed in the exposed. Comet assay data showed decreased TL values in the exposed population with respect to the control group, this might indicate the induction of crosslinks by the substances present in the workplace environment. Significant increase in MN frequency was obtained for GSTT1 null exposed individuals with respect to
Keyphrases
rubber tyre occupational genotoxic risk rubber worker gstt1 deletion polymorphism cyp2e1 dra workplace environment portuguese factory gstt1 null broad spectrum substance present control group general biomarker genetic polymorphism comet assay urinary thioethers peripheral blood sample certain form decreased tl value non-significant increase inmn frequency susceptibility biomarkers genotoxic effect exposed worker exposed population mn frequency sister chromatid exchange significant increase rubber industry
