DMCA
Ex Vivo Observation of Human Nucleus Pulposus Chondrocytes Isolated From Degenerated Intervertebral Discs
BibTeX
@MISC{Wang_exvivo,
author = {Feng Wang and Xiao-tao Wu and Su-yang Zhuang and Yun-tao Wang and Xing Hong and Lei Zhu and Jun-ping Bao},
title = {Ex Vivo Observation of Human Nucleus Pulposus Chondrocytes Isolated From Degenerated Intervertebral Discs},
year = {}
}
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Abstract
Study Design: We performed an ex vivo study to observe cell morphology and viability of human nucleus pulposus (NP) chondrocytes isolated from degenerated intervertebral discs (IVD). Purpose: To better understand the biological behavior of NP chondrocytes in monolayer cultures. Overview of Literature: Biological repair of IVDs by cell-based therapy has been shown to be feasible in clinical trials. As one of the most promising transplanting seeds, how the isolated NP chondrocytes behavior ex vivo has not been fully understood. Methods: Human NP chondrocytes were harvested from 20 degenerated IVDs and cultured in monolayers. Histological and immunochemistry staining was used to detect cell morphology change. Cell viability was studied by analyzing cell cycle distribution and apoptotic rate in the primary and subculuted cells. Results: The round or polygonal primary NP chondrocytes had an average adherence time of 7 days and took nearly 31 days to reach 95 % confluence. The spindle-shaped P1 NP chondrocytes increased growth kinetics and took about 12 hours to adhere and 6.6 days to get 95 % confluent. Immunochemistry staining of collagen II was positive in the cell cytoplasm. Nearly 90 % of the confluent NP chondrocytes stayed in G1 phase while 16 % underwent apoptosis. No significant difference of the collagen II expression, cell cycle distribution or the apoptosis indices were detected between the primary and subcultured NP chondrocytes. Conclusions: Human NP chondrocytes undergo significant morphological change in monolayer cultures. Cell cycle distribution pattern and apoptosis index of the cutured NP chondrocytes potentially influence their clinical transplantation or laboratory use.
Keyphrases
degenerated intervertebral disc human nucleus pulposus chondrocytes ex vivo observation apoptosis index np chondrocytes cell cycle distribution immunochemistry staining monolayer culture significant difference collagen ii expression cutured np cell-based therapy undergo significant morphological change biological behavior ex vivo study biological repair isolated np degenerated ivds clinical trial average adherence time cell cycle distribution pattern apoptotic rate cell cytoplasm study design subculuted cell growth kinetics clinical transplantation collagen ii human np promising transplanting seed spindle-shaped p1 np chondrocytes g1 phase human nucleus pulposus confluent np polygonal primary np chondrocytes laboratory use cell morphology underwent apoptosis human np chondrocytes cell morphology change cell viability
