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Short Communication ANTAGONISM OF DRUGS USED IN LEUKAEMIA THERAPY TO THE KILLING OF HUMAN LYMPHOBLASTOID CELLS BY
BibTeX
@MISC{Gledhill_shortcommunication,
author = {R. M. Gledhill and M. R. Norman and The Human and Cell Line Ccrf-cem},
title = {Short Communication ANTAGONISM OF DRUGS USED IN LEUKAEMIA THERAPY TO THE KILLING OF HUMAN LYMPHOBLASTOID CELLS BY},
year = {}
}
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Abstract
was derived by Foley et al. (1965) from the peripheral blood of a patient with acute lymphoblastic leukaemia (ALL). When tested by Norman & Thompson (1977) CCRF-CEM was only partially sensitive to glucocorticoids, but Clone C7, isolated from the parental cell line, was lysed by pharmacological concentrations of glucocorticoids. The availability of a steroid-sensitive human lymphoid cell line made it possible to study interactions between steroids and other chemotherapeutic drugs under conditions more appropriate to their clinical usage. Some preliminary results obtained with CEM-C7 cells have already been described (Norman et al., 1 978). This paper presents the results of further investigations into the interaction of prednisolone with drugs used in the treatment of ALL. The drugs used were 6-mercaptopurine (MP), methotrexate (MTX), cytosine arabinoside (Ara-C) and daunomycin (DMN). CEM-C7 cells were maintained in continuous culture in Medium RPMI 1640 supplemented with 10 % foetal calf serum (Flow Laboratories). In fresh medium cell growth was exponential between 105 and 5 x 106 cells/ml. Solutions of prednisolone and other drugs (Sigma Chemical Co.) were prepared fresh for each experiment. Prednisolone was added to cell suspensions from a stock solution in 10 % or 1% ethanol; an equivalent volume of ethanol was added to control cells. Ara-C and
Keyphrases
cem-c7 cell peripheral blood fresh medium cell growth chemotherapeutic drug norman thompson equivalent volume clinical usage parental cell line foetal calf serum cytosine arabinoside preliminary result pharmacological concentration clone c7 continuous culture steroid-sensitive human lymphoid cell line flow laboratory sigma chemical co medium rpmi acute lymphoblastic leukaemia stock solution
