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Targeted mutagenesis by homologous recombination in D. melanogaster. Genes Dev (2002)
| Citations: | 31 - 2 self |
BibTeX
@MISC{Rong02targetedmutagenesis,
author = {Yikang S. Rong and Simon W. Titen and Heng B. Xie and Mary M. Golic and Michael Bastiani and Pradip B and Baldomero M. Olivera and Michael Brodsky and Gerald M. Rubin},
title = {Targeted mutagenesis by homologous recombination in D. melanogaster. Genes Dev},
year = {2002}
}
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Abstract
We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate targeted alterations of the host genome. The results address several questions about the general utility of this technique. We show that genes not near telomeres can be efficiently targeted; that no knowledge of the mutant phenotype is needed for targeting; and that insertional mutations and allelic substitutions can be easily produced. [Key Words: Gene targeting; Drosophila; recombination; FLP; I-SceI]
Keyphrases
homologous recombination gene dev result address several question transgenic expression key word flp site-specific recombinase allelic substitution i-scei endonuclease insertional mutation general utility targeted alteration mutant phenotype extrachromosomal linear dna molecule p53 tumor suppressor host genome chromosomal locus endogenous drosophila gene mutant allele
