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Comparative phylogenetic analysis reveals multiple non-imprinted isoforms of opossum Dlk1 (2006)
| Venue: | Mamm. Genome |
| Citations: | 3 - 0 self |
BibTeX
@ARTICLE{Weidman06comparativephylogenetic,
author = {Jennifer R. Weidman and Kristin A. Maloney and Y L. Jirtle},
title = {Comparative phylogenetic analysis reveals multiple non-imprinted isoforms of opossum Dlk1},
journal = {Mamm. Genome},
year = {2006},
pages = {157--167}
}
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Abstract
Imprinted genes are monoallelically expressed in a parent-of-origin manner and were previously identi-fied in both marsupials and eutherians, but not in monotremes. Phylogenetic comparison of imprinted domains is a powerful tool for investigating the molecular and adaptive evolution of this unique gene regulatory mechanism. Herein, we report that mul-tiple transcripts of Dlk1 (Delta, Drosophila, Homo-log-like 1) are expressed in the opossum, but none are imprinted. Thus, we provide the first example of a reciprocally imprinted gene domain in which imprinting evolved in a common ancestor to euthe-rian rather than therian mammals. Moreover, the reciprocally imprinted Meg3 (Maternally Expressed Gene 3), found downstream of Dlk1 in eutherian mammals, is absent in the opossum. We propose that the Meg3 sequence integrated into the eutherian Dlk1 domain via a LINE-1 element and that Dlk1 became imprinted in eutherian mammals only after this downstream integration. These findings clearly demonstrate that imprinted genes did not all evolve before the divergence of marsupials and eutherians.
Keyphrases
opossum dlk1 eutherian mammal imprinted domain gene domain adaptive evolution downstream integration line-1 element eutherian dlk1 domain phylogenetic comparison parent-of-origin manner first example meg3 sequence unique gene regulatory mechanism powerful tool common ancestor mul-tiple transcript therian mammal imprinted gene
