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Pronounced segregation of donor mitochondria introduced by bovine ooplasmic transfer to the female germ-line (2010)
| Venue: | Biol. Reprod |
| Citations: | 1 - 1 self |
BibTeX
@ARTICLE{Ferreira10pronouncedsegregation,
author = {Christina Ramires Ferreira and Jörg Patrick Burgstaller and Felipe Perecin and Joaquim Mansano Garcia and Marcos Roberto Chiaratti and Simone Cristina Méo and Lawrence Charles Smith and Flávio Vieira Meirelles and Ralf Steinborn},
title = {Pronounced segregation of donor mitochondria introduced by bovine ooplasmic transfer to the female germ-line},
journal = {Biol. Reprod},
year = {2010},
pages = {563--571}
}
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Abstract
Ooplasmic transfer (OT) has been used in basic mouse research for studying the segregation of mtDNA, as well as in human assisted reproduction for improving embryo develop-ment in cases of persistent developmental failure. Using cattle as a large-animal model, we demonstrate that the moderate amount of mitochondria introduced by OT is transmitted to the offspring’s oocytes; e.g., modifies the germ line. The donor mtDNA was detectable in 25 % and 65 % of oocytes collected from two females. Its high variation in heteroplasmic oocytes, ranging from 1.1 % to 33.5 % and from 0.4 % to 15.5%, can be explained by random genetic drift in the female germ line. Centrifugation-mediated enrichment of mitochondria in the pole zone of the recipient zygote’s ooplasm and its substitution by donor ooplasm led to elevated proportions of donor mtDNA in
