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Generation of Chimeric Rhesus Monkeys
| Citations: | 6 - 0 self |
BibTeX
@MISC{Tachibana_generationof,
author = {Masahito Tachibana and Michelle Sparman and Cathy Ramsey and Hong Ma and Hyo-sang Lee and Maria Cecilia T. Penedo and Shoukhrat Mitalipov},
title = {Generation of Chimeric Rhesus Monkeys},
year = {}
}
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Abstract
Totipotent cells in early embryos are progenitors of all stem cells and are capable of developing into a whole organism, including extraembryonic tissues such as placenta. Pluripotent cells in the inner cell mass (ICM) are the descendants of totipotent cells and can differentiate into any cell type of a body except extraembryonic tissues. The ability to contribute to chimeric animals upon reintroduction into host embryos is the key feature of murine totipotent and pluripotent cells. Here, we demonstrate that rhesus monkey embryonic stem cells (ESCs) and isolated ICMs fail to incorporate into host embryos and develop into chimeras. However, chimeric offspring were produced following aggregation of totipotent cells of the four-cell embryos. These results provide insights into the species-specific nature of primate embryos and suggest that a chimera assay using pluripotent cells may not be feasible.
Keyphrases
chimeric rhesus monkey totipotent cell pluripotent cell extraembryonic tissue host embryo key feature whole organism chimera assay rhesus monkey embryonic stem cell primate embryo chimeric animal species-specific nature stem cell four-cell embryo isolated icms fail early embryo cell type murine totipotent inner cell mass
