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...uilt equipment. As discussed above, the effective conductivity and permittivity of the whole cell will be Journal of Biomedicine and Biotechnology 5 a function of the N/C ratio. The ability to characterise and separate cells on the basis of the fxo2 DEP cross-over frequency could therefore be particularly important for stem cell research, because the nucleus-to-cytoplasm (N/C) ratio generally decreases with cell differentiation and maturity [21]. For example, limbal epithelial stem cells exhibit an N/C ratio of 0.82, whilst for peripheral corneal epithelial cells the N/C ratio reduces to 0.17 [22]. DEP thus offers many distinct advantages as a tool for biomedical applications. In summary, these include the ability to: (i) monitor changes in cell viability, (ii) isolate viable, culturable, cells with minimal or no biological damage, (iii) monitor changes in the surface morphology or internal structure of cells, (iv) separate cells to high specificity for their identification and enumeration, (v) separate cells without the need for biochemical labelling or modification, (vi) separate rare target cells from heterogeneous samples, avoiding cell loss with a process that uses one procedure (...

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...eases with cell differentiation and maturity [21]. For example, limbal epithelial stem cells exhibit an N/C ratio of 0.82, whilst for peripheral corneal epithelial cells the N/C ratio reduces to 0.17 =-=[22]-=-. DEP thus offers many distinct advantages as a tool for biomedical applications. In summary, these include the ability to: (i) monitor changes in cell viability, (ii) isolate viable, culturable, cell...

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...n et al., 2004; Dua et al., 2003) and in rats (Chee et al., 2006). However, limbal epithelial cells expressing high levels of p63 with a high nuclear to cytoplasmic ratio appear to be more stem like (=-=Arpitha et al., 2005-=-). Further work has since indicated that the Np63α isoform may more specifically label LESC (Di Iorio et al., 2005). Many types of organ-specific stem cells, including LESC have been recently shown t...

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...ransient amplifying cells at various stages of maturity. LESCs are identified by their elevated expression of an isoform of the transcription factor p63 along with a high nuclear to cytoplasmic ratio =-=[7,8]-=-. ABCG2 (ATP binding cassette sub family G member 2) positivity has been detected in LESCs as well as several other cells residing in the suprabasal limbus and these markers have the potential to iden...