Results 1 - 10 of 17,397

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector

by Luigi Naldini, Ulrike Blomer, Daniel Ory, Richard Mulligan, Fred H. Gage, Inder M. Verma, Didier Trono - Science , 1996
"... A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additi ..."
Abstract - Cited by 478 (17 self) - Add to MetaCart
. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy. Until now, gene therapy protocols

Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery

by Romain Zufferey, Thomas Dull, Ronald J. M, Dulce Quiroz, Luigi Naldini, Didier Trono, Updated Information, Romain Zufferey, Thomas Dull, Ronald J. Mandel, Anatoly Bukovsky, Dulce Quiroz, Luigi Naldini, Didier Trono - J. Virol , 1998
"... These include: This article cites 31 articles, 21 of which can be accessed free at: ..."
Abstract - Cited by 140 (7 self) - Add to MetaCart
These include: This article cites 31 articles, 21 of which can be accessed free at:

Correspondence

by H. A. Lankes, C. N. Zanghi, K. Santos, C. Capella, C. M. P. Duke, S. Dewhurst, Stephen Dewhurst, Department Of
"... vivo gene delivery and expression by bacteriophage lambda vectors ..."
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vivo gene delivery and expression by bacteriophage lambda vectors

Robust Cardiomyocyte-Specific Gene Expression Following Systemic Injection of AAV: In Vivo Gene Delivery Follows a Poisson Distribution

by Konkal-matt R. Prasad, Brent A French
"... Newly-isolated serotypes of AAV readily cross the endothelial barrier to provide efficient transgene delivery throughout the body. However, tissue-specific expression is preferred in most experimental studies and gene therapy protocols. Previous efforts to restrict gene expression to the myocardium ..."
Abstract - Cited by 1 (0 self) - Add to MetaCart
Newly-isolated serotypes of AAV readily cross the endothelial barrier to provide efficient transgene delivery throughout the body. However, tissue-specific expression is preferred in most experimental studies and gene therapy protocols. Previous efforts to restrict gene expression to the myocardium

Biodegradable Poly(2-Dimethylamino Ethylamino)Phosphazene for In Vivo Gene Delivery to Tumor Cells. Effect of Polymer Molecular Weight

by Holger K. De Wolf, Markus De Raad, Cor Snel, Mies J. Van Steenbergen, Marcel H. A. M. Fens, Gert Storm, Wim E. Hennink , 2007
"... Purpose. Previously, we have shown that complexes of plasmid DNA with the biodegradable polymer poly(2-dimethylamino ethylamino)phosphazene (p(DMAEA)-ppz) mediated tumor selective gene expression after intravenous administration in mice. In this study, we investigated the effect of p(DMAEA)-ppz mole ..."
Abstract - Cited by 3 (0 self) - Add to MetaCart
(DMAEA)-ppz polyplexes for in vitro and in vivo tumor transfection. KEY WORDS: biodegradable; cationic polymer; DNA; molecular weight; tumor gene delivery.

In Vivo Gene Delivery and Visualization of Corneal Stromal Cells Using an Adenoviral Vector and Keratocyte-Specific Promoter

by Eric C. Carlson, Chia-yang Liu, Xiaoping Yang, Meredith Gregory, Bruce Ks, Judith Drazba, Victor L. Perez
"... PURPOSE. This study was conducted to determine whether intrastromal injection of adenoviral construct could be used to transfect corneal stroma cells effectively in vivo and to determine whether a tissue-specific promoter could be used to express exogenous genes in keratocytes. METHODS. An adenovira ..."
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PURPOSE. This study was conducted to determine whether intrastromal injection of adenoviral construct could be used to transfect corneal stroma cells effectively in vivo and to determine whether a tissue-specific promoter could be used to express exogenous genes in keratocytes. METHODS

Ž.

by Yi Liu A, B. Timothy Himes A, Jon Moul A, Wenlin Huang A, Stella Y. Chow A, Alan Tessler A, Itzhak Fischer A , 1997
"... Application of recombinant adenovirus for in vivo gene delivery to spinal cord ..."
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Application of recombinant adenovirus for in vivo gene delivery to spinal cord

Reducing Amyloid Plaque Burden via Ex Vivo Gene Delivery of an Ab-Degrading Protease: A Novel Therapeutic Approach to Alzheimer Disease

by Citable Link, Matthew L. Hemming, Michaela Patterson, Ling Lin, Ole Isacson, Dennis J. Selkoe , 2016
"... (Article begins on next page) The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters. ..."
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(Article begins on next page) The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters.

Research Article Preparation of a Nanoscaled Poly(vinyl alcohol)/ Hydroxyapatite/DNA Complex Using High Hydrostatic Pressure Technology for In Vitro and In Vivo Gene Delivery

by Tsuyoshi Kimura, Yoichi Nibe, Seiichi Funamoto, Masahiro Okada, Tsutomu Furuzono, Tsutomu Ono, Hidekazu Yoshizawa, Toshiya Fujisato, Kwangwoo Nam, Akio Kishida
"... Copyright © 2011 Tsuyoshi Kimura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Our previous research showed that poly(vinyl ..."
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Copyright © 2011 Tsuyoshi Kimura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Our previous research showed that poly(vinyl alcohol) (PVA) nanoparticles incorporating DNA with hydrogen bonds obtained by high hydrostatic pressurization are able to deliver DNA without any significant cytotoxicity. To enhance transfection efficiency of PVA/DNA nanoparticles, we describe a novel method to prepare PVA/DNA nanoparticles encapsulating nanoscaled hydroxyapatites (HAps) prepared by high hydrostatic pressurization (980 MPa), which is designed to facilitate endosomal escape induced by dissolving HAps in an endosome. Scanning electron microscopic observation and dynamic light scattering measurement revealed that HAps were significantly encapsulated in PVA/HAp/DNA nanoparticles. The cytotoxicity, cellular uptake, and transgene expression of PVA/HAp/DNA nanoparticles were investigated using COS-7 cells. It was found that, in

EX VIVO GENE DELIVERY OF GDNF USING PRIMARY ASTROCYTES TRANSDUCED WITH A LENTIVIRAL VECTOR PROVIDES NEUROPROTECTION IN A RAT MODEL OF PARKINSON´S DISEASE

by Ericson C, Georgievska B, Lundberg C, Cecilia Ericson, Biljana Georgievska, Cecilia Lundberg
"... Journal of Neuroscience. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination. Citation for the published paper: ..."
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Journal of Neuroscience. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination. Citation for the published paper:
Results 1 - 10 of 17,397