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Results 1 - 10
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1,437
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
- Science
, 1996
"... A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additi ..."
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Cited by 478 (17 self)
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A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages
Phenotypes of c-Mycdeficient rat fibroblasts isolated by targeted homologous recombination
- Cell Growth Differ
, 1997
"... Rat fibroblast cell lines with targeted disruptions of both c-myc gene copies were constructed. Although c-myc null cells are viable, their growth is significantly impaired. The absence of detectable N-myc or L-myc expression indicates that Myc function is not absolutely essential for cell viability ..."
Abstract
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Cited by 48 (5 self)
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Rat fibroblast cell lines with targeted disruptions of both c-myc gene copies were constructed. Although c-myc null cells are viable, their growth is significantly impaired. The absence of detectable N-myc or L-myc expression indicates that Myc function is not absolutely essential for cell
Entrainment of Spontaneously Hypertensive Rat Fibroblasts by Temperature Cycles
, 2013
"... The functional state of the circadian system of spontaneously hypertensive rats (SHR) differs in several characteristics from the functional state of normotensive Wistar rats. Some of these changes might be due to the compromised ability of the central pacemaker to entrain the peripheral clocks. Dai ..."
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of SHR results from a compromised peripheral clock sensitivity to the daily temperature cycle resetting. Using cultured Wistar rat and SHR fibroblasts transfected with the circadian luminescence reporter Bmal1-dLuc, we demonstrated that two consecutive square-wave temperature cycles with amplitudes of 2
Biological Similarities of Rat Fibroblast Interferon to Human and Mouse Alpha Interferons
, 1985
"... High titres of rat interferon (IFN) can be produced by rat embryo fibroblast (CD) cells after treatment with Newcastle disease virus. This CD IFN was characterized by SDS PAGE, and was found to contain three species at 30K to 33K, 25K to 27K and 17K to 22K mol. wt. Antibody affinity chromatography r ..."
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High titres of rat interferon (IFN) can be produced by rat embryo fibroblast (CD) cells after treatment with Newcastle disease virus. This CD IFN was characterized by SDS PAGE, and was found to contain three species at 30K to 33K, 25K to 27K and 17K to 22K mol. wt. Antibody affinity chromatography
Appearance of Intranuclear Actin Filaments in Rat Fibroblasts after Heat-Shock Treatment
"... ABSTRACT Using both electron microscopy and immunological methods, we have characterized a number of changes occurring in rat fibroblasts after heat-shock treatment. Incubation of the cells for 3 h at 42°-43°C resulted in a number of changes within the cytoplasm including: a disruption and fragmenta ..."
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ABSTRACT Using both electron microscopy and immunological methods, we have characterized a number of changes occurring in rat fibroblasts after heat-shock treatment. Incubation of the cells for 3 h at 42°-43°C resulted in a number of changes within the cytoplasm including: a disruption
Mitochondrial function in oncogene-transfected rat fibroblasts isolated from multicellular spheroids
- Am. J. Physiol
, 1997
"... fibroblasts isolated from multicellular spheroids. Am. J. Physiol. 273 (Cell Physiol. 42): C1487–C1495, 1997.—Two mitochondrion-specific fluorochromes, 10-N-nonyl acridine orange (NAO) and rhodamine 123 (Rh123), were used to determine the mechanism responsible for alterations in en-ergy metabolism o ..."
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Cited by 1 (0 self)
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of transformed rat embryo fibroblast cells isolated from different locations within multicellular spher-oids. Accumulation of Rh123 depends on intact mitochondrial membrane potential, whereas NAO is taken up by mitochon-dria independently of their function and thus represents mitochondrial distribution only
Printed in Great Britain ~89 In vitro Transformation of Primary and Continuous Rat Fibroblasts by Rous Sarcoma Virus (SCHMIDT-RUPPIN)
"... In vitro transformation of rat fibroblasts by the SCHMIDT-RUPPIN strain of Rous sarcoma virus is described. Primary rat embryo fibroblasts were transformed in 2 to 6 weeks after infection with the virus. The transformed cells were large, refractile and polygonal in appearance and produced multilayer ..."
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In vitro transformation of rat fibroblasts by the SCHMIDT-RUPPIN strain of Rous sarcoma virus is described. Primary rat embryo fibroblasts were transformed in 2 to 6 weeks after infection with the virus. The transformed cells were large, refractile and polygonal in appearance and produced
LOSS OF DNA REPAIR CAPACITY DURING SUCCESSIVE SUBCULTURES OF PRIMARY RAT FIBROBLASTS
"... Cultures of fibroblasts from newborn rats and successive subcultures of these cells were treated with 4-nitroquinoline-l-oxide to induce DNA repair. DNA from the cultures was examined by velocity sedimentation in alkaline sucrose gradients immediately after drug treatment and after a post-treatment ..."
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Cultures of fibroblasts from newborn rats and successive subcultures of these cells were treated with 4-nitroquinoline-l-oxide to induce DNA repair. DNA from the cultures was examined by velocity sedimentation in alkaline sucrose gradients immediately after drug treatment and after a post
Continuous production of minute virus of mice by an untransformed variant of Fisher rat fibroblast (FR3T3)
"... Many tumour cells are killed by the lytic replication of the autonomous parvoviruses H-I and minute virus of mice (MVMp), whereas most untransformed cells (although they take up these viruses efficiently) are resistant, i.e. they do not produce infectious virus and are not lysed. Therefore, cells ab ..."
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Cited by 1 (1 self)
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able to continuously produce large quantities of infectious virus have not yet been described. We have isolated such cells from the resistant cell line FR3T3 (Fisher rat fibroblast). These cells (called FR3T3 C) produce infectious MVMp virions without being detectably lysed. Furthermore, a per
840) VARIATIONS IN THE TUMOUR-FORMII:NG CAPACITY OF A LINE OF RAT FIBROBLASTS (16C) FOLLOWING SELECTIONT IN VITRO
, 1965
"... THE Warburg hypothesis of malignant change proposed that ain irreversible respiratory lesion in a cell is followed by a compensating increase in the ability to derive energy by the glycolytic breakdown of glucose. This process is assumed to be unaffected by the mechanism of respiratory inhibition, w ..."
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produced no tumours. This finding appeared to support the Warburg theory, but many reports now confirm that such a transformation in vitro may occur in both mouse and rat fibroblasts in normal aerobic culture without the demonstrable intervention of any carcinogenic agent (Gey.
Results 1 - 10
of
1,437
