Results 1 - 10 of 3,477

In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector

by Luigi Naldini, Ulrike Blomer, Daniel Ory, Richard Mulligan, Fred H. Gage, Inder M. Verma, Didier Trono - Science , 1996
"... A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additi ..."
Abstract - Cited by 478 (17 self) - Add to MetaCart
. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy. Until now, gene therapy protocols

Quantification of reverse transcriptase activity by real-time PCR as a fast and accurate method for titration of HIV, lenti- and retroviral vectors. PLoS One 7: e50859

by Jolien Vermeire, Evelien Naessens, Hanne V, Alessia L, Veronica Iannucci, Anouk Van Nuffel, Tom Taghon, Massimo Pizzato, Bruno Verhasselt , 2012
"... Quantification of retroviruses in cell culture supernatants and other biological preparations is required in a diverse spectrum of laboratories and applications. Methods based on antigen detection, such as p24 for HIV, or on genome detection are virus specific and sometimes suffer from a limited dyn ..."
Abstract - Cited by 3 (0 self) - Add to MetaCart
-PERT values correlate with transducing and infectious units in HIV-based viral vector and replication-competent HIV-1 preparations respectively. This assay can furthermore quantify Moloney Murine Leukemia Virus-derived vectors and can be performed on different instruments, such as Roche LightcyclerH 480

Global analysis of cellular transcription following infection with an HIV-based vector. Mol. Ther

by Richard Mitchell, Chih-yuan Chiang, Charles Berry, Frederic Bushman , 2003
"... We have examined the changes in cellular transcription resulting from infection with HIV-based vectors. Previous work suggested that the incoming viral genome may under some circumstances be detected as DNA damage, so to explore this possibility, we compared the transcriptional response to infection ..."
Abstract - Cited by 10 (0 self) - Add to MetaCart
We have examined the changes in cellular transcription resulting from infection with HIV-based vectors. Previous work suggested that the incoming viral genome may under some circumstances be detected as DNA damage, so to explore this possibility, we compared the transcriptional response

Declassification of Rodents Exposed to Third-Generation HIV-Based Vectors into Class 1 Animals

by Stéphane Karlen, Romain Zufferey
"... According to national and international regulations, experiments involving large amount of HIV-1 are generally restricted to infrastructures with a Biosafety Level 3 (BSL-3) status. However, work with genetically modified viral vectors such as the replication-defective lentiviral vectors developed b ..."
Abstract - Cited by 1 (0 self) - Add to MetaCart
According to national and international regulations, experiments involving large amount of HIV-1 are generally restricted to infrastructures with a Biosafety Level 3 (BSL-3) status. However, work with genetically modified viral vectors such as the replication-defective lentiviral vectors developed

Intravenous Delivery of HIV-Based Lentiviral Vectors Preferentially Transduces F4/80+ and Ly-6C+ Cells in Spleen, Important Target Cells in Autoimmune Arthritis

by Ben T. Van Den Br, Eline A. Vermeij, Claire E. J. Waterborg, Onno J. Arntz, Michael Kracht, A B. Bennink, Wim B. Van Den Berg
"... Antigen presenting cells (APCs) play an important role in arthritis and APC specific gene therapeutic targeting will enable intracellular modulation of cell activity. Viral mediated overexpression is a potent approach to achieve adequate transgene expression levels and lentivirus (LV) is useful for ..."
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Antigen presenting cells (APCs) play an important role in arthritis and APC specific gene therapeutic targeting will enable intracellular modulation of cell activity. Viral mediated overexpression is a potent approach to achieve adequate transgene expression levels and lentivirus (LV) is useful

Supplemental Experimental Procedures Construction of Viral Vectors

by Bryan E. Welm, Gerrit J. P. Dijkgraaf, Anita S. Bledau, Alana L. Welm, Zena Werb
"... MMLV-EGFP is a self-inactivating retrovirus that was created by replacing the CMVie-MCS-IRES-Neo r content of pQCXIN (Clontech) with a cassette consisting of the EF1α (Kim et al., 1990) promoter fused to EGFP (Cormack et al., 1996). The U6-TATAlox-CMVie-EGFP-TATAlox-WPRE content of pSICO (Ventura et ..."
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et al., 2004) was removed and the resulting empty viral vector served as a backbone for all HIV-based selfinactivating lentiviral vectors described below. Insertion of the EF1α-EGFP cassette into this backbone created HIV-EGFP. Replacing EGFP with a fragment containing a multiple cloning site (MCS

Efficient Transduction of Pigtailed Macaque Hematopoietic Repopulating Cells with HIV-based Lentiviral Vectors

by Grant D. Trobridge, Brian C. Beard, Christina Gooch, Martin Wohlfahrt, Philip Olsen, James Fletcher, Punam Malik, Hans-peter Kiem, Gene Therapy Articles, Grant D. Trobridge, Brian C. Beard, Christina Gooch, Martin Wohlfahrt, Philip Olsen, James Fletcher, Punam Malik, Hans-peter Kiem , 2008
"... only. ..."
Abstract - Cited by 2 (0 self) - Add to MetaCart
Abstract not found

ARTICLE doi:10.1016/j.ymthe.2005.10.009 Integration Site Selection by HIV-Based Vectors in Dividing

by Growth-arrested Imr, Lung Fibroblasts, Angela Ciuffi, Richard S. Mitchell, Christian Hoffmann, Jeremy Leipzig, Paul Shinn, Joseph R. Ecker, Frederic D. Bushman Y , 2005
"... *These authors contributed equally to this work. y To whom correspondence and reprint requests should be addressed. ..."
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*These authors contributed equally to this work. y To whom correspondence and reprint requests should be addressed.

Development of a self-inactivating lentivirus vector

by Hiroyuki Miyoshi, Masayo Takahashi, Fred H. Gage, Inder, M. Verma - J Virol , 1998
"... We have constructed a new series of lentivirus vectors based on human immunodeficiency virus type 1 (HIV-1) that can transduce nondividing cells. The U3 region of the 5 * long terminal repeat (LTR) in vector constructs was replaced with the cytomegalovirus (CMV) promoter, resulting in Tat-independen ..."
Abstract - Cited by 140 (9 self) - Add to MetaCart
We have constructed a new series of lentivirus vectors based on human immunodeficiency virus type 1 (HIV-1) that can transduce nondividing cells. The U3 region of the 5 * long terminal repeat (LTR) in vector constructs was replaced with the cytomegalovirus (CMV) promoter, resulting in Tat

Virus-Based Retroviral Vector: Recovery of an Anti-HIV

by Mouse/human Pseudotype Retrovirus, M. Federico, F. Nappi, G. Ferrari, C. Chelucci, F. Mavilio, P. Verani , 1995
"... The expression of a human immunodeficiency virus (HIV) type 1 provirus (F12-HIV) cloned from a nonproducer, chronically infected CD4 down-regulated Hut-78 cell clone (F12) does not lead to the formation of viral particles and, upon transfection in HeLa CD41 cells, confers resistance to HIV superinfe ..."
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superinfection without affecting the CD4 receptor exposure. In an attempt to transfer the anti-HIV properties of F12-HIV into human primary cells, we constructed a Moloney murine leukemia virus-based retroviral vector containing an F12-HIV genome lacking the 3 * long terminal repeat and part of the nef gene
Results 1 - 10 of 3,477