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Results 1 - 10
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912
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
- Science
, 1996
"... A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additi ..."
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Cited by 478 (17 self)
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A retroviral vector system based on the human immunodeficiency virus (HIV) was de-veloped that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages
Removal of Heterologous Sequences from Plasmodium falciparum Mutants Using FLPe-Recombinase
, 2010
"... Genetically-modified mutants are now indispensable Plasmodium gene-function reagents, which are also being pursued as genetically attenuated parasite vaccines. Currently, the generation of transgenic malaria-parasites requires the use of drugresistance markers. Here we present the development of an ..."
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that has a selectable marker cassette flanked by FRT-sequences. This permitted the subsequent removal of the selectable marker cassette by transient transfection of a plasmid that expressed a 37uC thermostable and enhanced FLPrecombinase. This method of removing heterologous DNA sequences from the genome
Branch migration through dna sequence heterology
, 1998
"... General genetic recombination and the recombinational repair of DNA damage are highly conserved processes that play essential roles in regulating the diversity and integrity of the genome. ..."
Abstract
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Cited by 5 (0 self)
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General genetic recombination and the recombinational repair of DNA damage are highly conserved processes that play essential roles in regulating the diversity and integrity of the genome.
expression of heterologous proteins
"... Background: Many plasmid-harbouring strains of Lactococcus lactis have been isolated from milk and other sources. Plasmids of Lactococcus have been shown to harbour antibiotic resistance genes and those that express some important proteins. The generally regarded as safe (GRAS) status of L. lactis a ..."
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of the strains was identified as a potential new lactococcal host for the expression of heterologous proteins. Results: Several bacterial strains were isolated from cow’s milk and eight of those were identified as Lactococcus lactis by 16S rRNA sequence analysis. Antibiotic susceptibility tests that were carried
Heterologous Transposon Tagging of the DRLl Locus in
"... The development of heterologous transposon tagging systems has been an important objective for many laboratories. Here, we demonstrate the use of a Dissociation (Ds) derivative of the maize transposable element Activator (Ac) to tag the D R L l locus of Arabidopsis. The drl7 mutant shows highly abno ..."
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Cited by 1 (0 self)
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The development of heterologous transposon tagging systems has been an important objective for many laboratories. Here, we demonstrate the use of a Dissociation (Ds) derivative of the maize transposable element Activator (Ac) to tag the D R L l locus of Arabidopsis. The drl7 mutant shows highly
Identification and Heterologous Expression of Genes Involved
, 2010
"... Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphi-toxidans, constructed using the fosmid vector pJK050, was screened for clones harb ..."
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harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino acid sequences of phosphite-induced proteins. Sequence analysis of two positive clones revealed a putative operon of seven genes predicted to be involved in phosphite oxidation. Four of these genes (ptx
Primary Sequence and Heterologous Expression of Nuclear Pore Glycoprotein p62
"... Abstract. The major nuclear pore protein p62 is modified by O-linked N-acetylglucosamine and functions in nuclear transport. We have cloned, sequenced, and expressed the full-length rat p62 cDNA. The rat p62 mRNA is 2,941 nucleotides long and encodes a protein of 525 amino acids containing 30 % seri ..."
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% serine and threonine residues. The amino acid sequence near the amino-terminus contains unique tetrapeptide repeats while the carboxy-terminus consists of a series of predicted alpha-helical regions with hydrophobic heptad repeats. Heterologous expression of rat p62 in African Green Monkey kidney COS
2003. Functional replacement of nucleocapsid flanking regions by heterologous counterparts with divergent primary sequences: effects of chimeric nucleocapsid on the retroviral replication cycle
- J
"... Nucleocapsid (NC) proteins in most retroviruses have a well-conserved Cys-His box(es) as well as more divergent flanking regions that are rich in basic residues. Mutations in the flanking regions can affect RNA packaging, virus assembly, and reverse transcription of the viral RNA. To gain a further ..."
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Cited by 9 (3 self)
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assembly. This finding indicates that, although the primary sequences of these flanking regions have little homology, the heterologous sequences are functional both as part of the Gag polyprotein and as processed NC protein. The gag gene in retroviruses encodes the structural proteins of the virions (60
Synonymous but not the same: The causes and consequences of codon bias.
- Nat Rev Genet
, 2010
"... When the inherent redundancy of the genetic code was discovered, scientists were rightly puzzled by the role of synonymous mutations 1 . The central dogma of molecular biology suggests that synonymous mutations -those that do not alter the encoded amino acid -will have no effect on the resulting pr ..."
Abstract
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Cited by 133 (0 self)
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protein sequence and, therefore, no effect on cellular function, organismal fitness or evolution. Nonetheless, in most sequenced genomes, synonymous codons are not used in equal frequencies. This phenomenon, termed codon-usage bias Advances in synthetic biology, mass spectrometry and sequencing now
Abstract The filamentous fungus Paecilomyces variotii
"... sequencing, and heterologous expression ..."
Results 1 - 10
of
912
