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200
unknown title
, 2011
"... Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting ..."
Abstract
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Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting
Relevance of Isogenicity for Targeting in Embryonic Stem
"... 258 PUBLICATIONS 14,806 CITATIONS ..."
Direct Cloning of Isogenic Murine DNA in Yeast and Relevance of Isogenicity for Targeting in Embryonic Stem
, 2013
"... Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here, ..."
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Efficient gene targeting in embryonic stem cells requires that modifying DNA sequences are identical to those in the targeted chromosomal locus. Yet, there is a paucity of isogenic genomic clones for human cell lines and PCR amplification cannot be used in many mutation-sensitive applications. Here
Multiplexed protein quantitation in Saccharomyces cerevisiae using aminereactive isobaric tagging reagents,”
- Molecular and Cellular Proteomics,
, 2004
"... We describe here a multiplexed protein quantitation strategy that provides relative and absolute measurements of proteins in complex mixtures. At the core of this methodology is a multiplexed set of isobaric reagents that yield amine-derivatized peptides. The derivatized peptides are indistinguisha ..."
Abstract
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Cited by 248 (1 self)
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are indistinguishable in MS, but exhibit intense low-mass MS/MS signature ions that support quantitation. In this study, we have examined the global protein expression of a wild-type yeast strain and the isogenic upf1⌬ and xrn1⌬ mutant strains that are defective in the nonsense-mediated mRNA decay and the general 5
Innodata Isogen White Paper SNAPCM
"... SnapCM is an abstract model for a Configuration Management and Version Control system which supports both time-based management of versioned resources and version-aware references. The management of versioned resources and versioned references can be very simple, or scale to arbitrary complexity by ..."
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time. Version-to-Version associations must be handled by versioning-aware References objects that can resolve (via extensible policy) the correct target Version. Versions that exist at the same time and are used in the same context are effective together this is referred to as a Snapshot of those
Exploration of essential gene functions via titratable promoter alleles. Cell 118:31–44
, 2004
"... lar biology, due in part to the ease of making targeted integrations in the genome. Recently, a consortium of laboratories created a collection of isogenic knockouts ..."
Abstract
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Cited by 98 (4 self)
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lar biology, due in part to the ease of making targeted integrations in the genome. Recently, a consortium of laboratories created a collection of isogenic knockouts
An Inducible, Isogenic Cancer Cell Line System for Targeting the State of Mismatch Repair Deficiency
, 2013
"... The DNA mismatch repair system (MMR) maintains genome stability through recognition and repair of single-base mismatches and small insertion-deletion loops. Inactivation of the MMR pathway causes microsatellite instability and the accumulation of genomic mutations that can cause or contribute to can ..."
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isogenic cancer cell lines for direct comparison of MMR-proficient and MMR-deficient cells. We engineered NCI-H23 lung adenocarcinoma cells to contain a doxycycline-inducible shRNA designed to suppress the expression of the mismatch repair gene MLH1, and compared single cell subclones that were uninduced
Reexamination of gene targeting frequency as a function of the extent of homology between the targeting vector and the target locus
- Mol. Cell. Biol
, 1992
"... Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two sites within the Hprt locus as a function of the extent of homology between the targeting vector and the ..."
Abstract
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Cited by 63 (4 self)
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and the target locus. The targeting frequency was also compared by using vectors prepared from isogenic and nonisogenic DNA sources. With one exception, all of the vectors showed the same exponential dependence of targeting efficiency on the extent of homology between the targeting vector and the target locus
Fitness of Isogenic Colony Morphology Variants of Pseudomonas aeruginosa in Murine Airway Infection
"... Chronic lung infections with Pseudomonas aeruginosa are associated with the diversification of the persisting clone into niche specialists and morphotypes, a phenomenon called ‘dissociative behaviour’. To explore the potential of P. aeruginosa to change its morphotype by single step loss-of–function ..."
Abstract
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Cited by 1 (1 self)
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of the 55 targets encode features of primary or secondary metabolism whereby quinolone production was frequently affected. In the other half the transposon had inserted into genes of the functional categories transport, regulation or motility/ chemotaxis. To mimic dissociative behaviour of isogenic strains
Evaluation of therapeutic measures for treating endophthalmitis caused by isogenic toxin-producing and toxin-nonproducing enterococcus faecalis strains
, 1995
"... Purpose. Management of endophthalmitis typically includes antibiotic combinations to arrest bacterial growth and antiinflammatory agents to limit inflammatory damage to sensitive tissues. Little research has been reported that systematically evaluates the contribution of each therapeutic component ..."
Abstract
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Cited by 11 (11 self)
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therapeutic targeting of the toxins involved. Invest Ophthalmol Vis Sci. 1995;36:9-15.
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