Abstract

The capacity of four culture media to obfuscate the antifungal activity of miconazole and amphotericin B methyl ester was evaluated qualitatively by examination of five isolates each of Candida albicans, Candida tropicalis, Candida parapsilosis, Toru-lopsis glabrata, and Cryptococcus neojormans, and quantitatively by determination of the absolute minimal inhibitory concentrations for a strain of C. albicans. Mi-conazole, like the predecessor imidazo.e (clotrimazole), was antagonized by two complex, undefined media (Sabouraud's glucose and brain-heart infusion agars) but not by either of two synthetic formulations (synthetic amino acid medium, fungal and modified yeast-nitrogen base). The antifungal activity of amphoteri-cin B methyl ester, like that of the parent compound amphotericin B, was not ma-terially affected by the culture medium used for testing. When added separately to synthetic amino acid medium (fungal), neither purines nor pyrimidines antagon-ized miconazole. Ether extraction of Sabouraud's glucose agar failed to diminish the antagonism of this medium for miconazole. Miconazole [1] and amphotericin B methyl ester