DMCA
Heparan Sulfate Motifs†,‡ (2001)
BibTeX
@MISC{Toida01heparansulfate,
author = {Toshihiko Toida and Mikio Kan and Huguette Pelletier and Wallace L. Mckeehan},
title = {Heparan Sulfate Motifs†,‡},
year = {2001}
}
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Abstract
ABSTRACT: Stromal cell-derived FGF-7 binds and activates only the resident FGFR2IIIb in epithelial cells while FGF-1 and FGF-2 exhibit a broader interaction with multiple isoforms of FGFR. Here we report the structure of FGF-7 that has been solved to 3.1 Å resolution by molecular replacement with the structure of a dual function chimera of FGF-7 and FGF-1 (FGF-7/1) which was resolved to 2.3 Å. Comparison of the FGF-7 structure to that of FGF-1 and FGF-2 revealed the strongly conserved CR backbone among the three FGF polypeptides and the surface hydrophobic patch that forms the primary receptor-binding domain. In contrast, a decrease and dispersion of the positive surface charge density characterized the heparin-
Keyphrases
heparan sulfate motif positive surface charge density stromal cell-derived fgf-7 bind fgf-2 exhibit primary receptor-binding domain molecular replacement dual function chimera fgf-7 structure epithelial cell fgf polypeptide multiple isoforms surface hydrophobic patch resident fgfr2iiib cr backbone
