DMCA
Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation (2006)
| Venue: | J. Immunol |
| Citations: | 70 - 0 self |
BibTeX
@ARTICLE{Blasius06bonemarrow,
author = {A L. Blasius and Emanuele Giurisato and Marina Cella and Robert D. Schreiber and Andrey S. Shaw and Marco Colonna},
title = {Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation},
journal = {J. Immunol},
year = {2006},
pages = {3260--3265}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
Type I IFN-producing cells (IPC) are sentinels of viral infections. Identification and functional characterization of these cells have been difficult because of their small numbers in blood and tissues and their complex cell surface phenotype. To overcome this problem in mice, mAbs recognizing IPC-specific cell surface molecules have been generated. In this study, we report the identi-fication of new Abs specific for mouse IPC, which recognize the bone marrow stromal cell Ag 2 (BST2). Interestingly, previously reported IPC-specific Abs 120G8 and plasmacytoid dendritic cell Ag-1 also recognize BST2. BST2 is predominantly specific for mouse IPC in naive mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-. The activation-induced promiscuous expression of BST2 described in this study has important implications for the use of anti-BST2 Abs in identification and depletion of IPC. Finally, we show that BST2 resides within an intracellular compartment corresponding to the Golgi apparatus, and may be involved in trafficking secreted cytokines in IPC. The Journal of Immunology, 2006, 177: 3260– 3265. T ype I IFN-producing cells (IPC),3 also called plasmacy-toid dendritic cells (DC), are early responders to viralinfections (1, 2). IPC detect viruses through TLRs 7 and 9 (3) and produce large amounts of type I IFN, i.e., IFN- and IFN- and proinflammatory chemokines. Through secretion of type I IFN, IPC direct both the innate and adaptive immune re-sponse by promoting NK cell and CD8 T cell cytotoxicity, en-hancing DC maturation, presenting Ag to T cells and inducing their Th1 polarization, and inducing B cell differentiation into Ab-secreting plasma cells (1, 2). IPC develop in the bone marrow and then circulate through the blood, eventually migrating into lym-phoid and nonlymphoid organs (1, 2), with increased recruitment into sites of inflammation (4, 5). Although IPC have significant effects on other cells, their numbers in blood and tissues are very small. This along with their complex surface phenotype makes them extremely difficult to identify. Specifically, mouse IPC are
Keyphrases
ifn-producing cell naive mouse promiscuous cell surface antigen ifn stimulation specific marker mouse ipc bone marrow stromal cell antigen ab-secreting plasma cell viral infection increased recruitment nonlymphoid organ functional characterization plasmacy-toid dendritic cell bst2 resides ipc-specific ab cd8 cell cytotoxicity en-hancing dc maturation significant effect th1 polarization golgi apparatus plasmacytoid dendritic cell ag-1 adaptive immune re-sponse ipc detect virus anti-bst2 ab early responder bone marrow stromal cell ag complex cell surface phenotype small number secreted cytokine ipc-specific cell surface molecule intracellular compartment bone marrow new ab specific activation-induced promiscuous expression cell differentiation proinflammatory chemokines nk cell large amount important implication complex surface phenotype cell type ipc develop
