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Culture Media for Large-scale Production of Micrococcin
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BibTeX
@MISC{Kelly_culturemedia,
author = {B. K. Kelly and G. A. Miller and C. W. Hale},
title = {Culture Media for Large-scale Production of Micrococcin},
year = {}
}
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Abstract
SUMMARY: A number of media have been developed for the production of micro-coccin. A satisfactory medium consists of corn steep liquor to 1800 mg. N/1. with an aqueous extract of dried autolysed yeast to 1800 mg. N/l., which gives titres of the order of 40-6Opg. micrococcin/ml. in 3 days. A soluble yeast extract such as Marmite may be used in place of the dried autolysed yeast. Moderate aeration is required for good titres but initial pH is relatively unimportant, any value in the range 6.0-7.0 being suitable. The main energy source in sugar-free media is probably the aerobic oxidative deamination of amino-acids. An inoculum that has been pre-incubated gives better results than an inoculum used immediately after it has been prepared. The addition of various inorganic ions to the medium was tried without any detectable influence on titre. The production of micrococcin (Su, 1948) in quantity was undertaken to provide material with which other workers could examine extraction of the active substance and its behaviour both in vitro and in vivo. The principal object of the present experiments was to reduce the cost of the medium and to
Keyphrases
large-scale production culture medium autolysed yeast satisfactory medium micrococcin ml moderate aeration various inorganic ion good titre corn steep liquor aerobic oxidative deamination aqueous extract detectable influence initial ph principal object present experiment soluble yeast extract sugar-free medium active substance main energy source
