DMCA
Highly efficient antigen targeting to M-DC81 dendritic cells via FccRIII/CD16-specific antibody conjugates
BibTeX
@MISC{Mende_highlyefficient,
author = {Ines Mende and Patrick Hoffmann and Andreas Wolf and Patrick A. Baeuerle and Annegret De Baey and Peter Kufer},
title = {Highly efficient antigen targeting to M-DC81 dendritic cells via FccRIII/CD16-specific antibody conjugates},
year = {}
}
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Abstract
Conjugates of peptide antigens with antibodies specifically recognizing surface molecules on dendritic cells (DC) represent an attractive approach to target antigens to antigen-presenting cells (APC) for the induction of specific T cell responses. The present study evaluates the potential of M-DC81 DC, a sub-population of professional APC in the blood, for an antibody-based vaccination strategy. We prepared, by chemical cross-linking, conjugates of peptide model antigens with antibodies directed against different cell surface molecules of DC. Antigen–peptide conjugates using an anti-CD16 (FccRIII) antibody were most potent in inducing in vitro activation of a specific CD41 T cell response. They were at least 300 times more efficient than two other antibody–antigen conjugates and ~500 times more efficient than unconjugated antigen peptides. Our data demonstrate that specific antigen targeting via CD16 on M-DC81 DC is a promising vaccination approach for the efficient induction of specific CD41 T cell responses ex vivo, and perhaps in vivo.
Keyphrases
fccriii cd16-specific antibody conjugate m-dc81 dendritic cell highly efficient antigen m-dc81 dc specific cd41 cell response surface molecule specific antigen dendritic cell peptide model antigen professional apc unconjugated antigen peptide peptide antigen data demonstrate attractive approach antigen-presenting cell specific cell response antibody antigen conjugate antibody-based vaccination strategy chemical cross-linking efficient induction promising vaccination approach present study antigen peptide vitro activation different cell surface molecule
