Mitotic crossovers between diverged sequences are regulated by mismatch repair proteins (1996)
| Venue: | in Saccharomyces |
| Citations: | 43 - 5 self |
BibTeX
@INPROCEEDINGS{Datta96mitoticcrossovers,
author = {Abhijit Datta and Adouda Adjiri and Liguo New and Gray F. Crouse},
title = {Mitotic crossovers between diverged sequences are regulated by mismatch repair proteins},
booktitle = {in Saccharomyces},
year = {1996},
pages = {1085--1093}
}
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Abstract
Mismatch repair systems correct replication- and recombination-associated mispaired bases and influence the stability of simple repeats. These systems thus serve multiple roles in maintaining genetic stability in eukaryotes, and human mismatch repair defects have been associated with hereditary predisposition to cancer. In prokaryotes, mismatch repair systems also have been shown to limit recombination between diverged (homeologous) sequences. We have developed a unique intron-based assay system to examine the effects of yeast mismatch repair genes (PMS1, MSH2, and MSH3) on crossovers between homeologous sequences. We find that the apparent antirecombination effects of mismatch repair proteins in mitosis are related to the degree of substrate divergence. Defects in mismatch repair can elevate homeologous recombination between 91 % homologous substrates as much as 100-fold while having only modest effects on recombination between 77 % homologous substrates. These observations have implications for genome stability and general mecha-nisms of recombination in eukaryotes. Much of our knowledge concerning the mechanism of ho-mologous recombination in eukaryotes has come from studies done with the yeast Saccharomyces cerevisiae (for a review, see reference 40), in which two basic types of recombination occur:
Keyphrases
mismatch repair protein mitotic crossover homologous substrate hereditary predisposition mismatch repair system human mismatch repair defect modest effect general mecha-nisms homeologous recombination genome stability homeologous sequence yeast saccharomyces cerevisiae unique intron-based assay system mismatch repair system correct replication multiple role ho-mologous recombination genetic stability simple repeat yeast mismatch repair gene basic type recombination occur apparent antirecombination effect mismatch repair substrate divergence
