SECRETORY POTENTIAL AND IONIC TRANSPORT IN THE POSTERIOR SILK GLANDS OF BOMBYX MORI (1987)
BibTeX
@MISC{Nakagaki87secretorypotential,
author = {I. Nakagaki and S. Sasaki},
title = {SECRETORY POTENTIAL AND IONIC TRANSPORT IN THE POSTERIOR SILK GLANDS OF BOMBYX MORI},
year = {1987}
}
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Abstract
of the posterior silk gland cells of Bombyx mori were measured by X-ray microprobe analysis of freeze-dried thin sections. The basal and luminal membrane potentials of the gland cells were measured using microelectrode techniques. The input resistance of the luminal plasma membrane was simultaneously measured by injecting electric current via an intracellular microelectrode. 2. The basolateral membrane potential was —47 ± l-8mV (s.E.) (iV = 46), and the glands exhibited lumen-negative voltages of —6 ± 0-1 mV (s.E.) (iY = 40) in the normal state. Increasing the extracellular K+ concentration depolarized the basolat-eral membrane potential, whereas the membrane potential hyperpolarized when Cl~ concentration in the extracellular fluid was increased. There were no significant effects on the membrane potential when Na+, Mg2+ and Ca2+ concentrations in the extracellular fluid were changed. 3. The representative X-ray spectra showed high K and phosphorus peaks, and low Cl and Mg peaks in the cytoplasm of the normal posterior silk gland cells. The normal glandular lumen showed relatively high K, and low Cl, sulphur, Ca and Mg peaks. Quantitative microprobe values were, for the cytoplasm (mmolkg"1 wet
Keyphrases
low cl membrane potential mg peak extracellular fluid 0-1 mv luminal membrane potential ca2 concentration microelectrode technique intracellular microelectrode significant effect gland cell luminal plasma membrane representative x-ray spectrum lumen-negative voltage cl concentration basolateral membrane potential freeze-dried thin section bombyx mori quantitative microprobe value normal posterior silk gland cell extracellular concentration normal glandular lumen normal state basolat-eral membrane potential posterior silk gland cell phosphorus peak x-ray microprobe analysis input resistance
