DMCA
Method for Regulated Expression of Single-Copy Efflux Pump Genes in a Surrogate Pseudomonas aeruginosa Strain: Identification of the (2006)
BibTeX
@MISC{Chloramphenicol06methodfor,
author = {Bpeef-oprc Chloramphenicol and Trimethoprim Efflux Pump and Ayush Kumar and Kim-lee Chua and Herbert P. Schweizer},
title = {Method for Regulated Expression of Single-Copy Efflux Pump Genes in a Surrogate Pseudomonas aeruginosa Strain: Identification of the},
year = {2006}
}
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Abstract
Construction and integration of recombinant mini-Tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe Pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. Using this method, the Burkholderia pseudomallei bpeEF-oprC operon was shown to encode a chloramphenicol and trimethoprim efflux pump. Multidrug resistance pumps play major roles in intrinsic and acquired bacterial antibiotic resistance and also in bacterial pathogenicity (13). A major handicap associated with the char-acterization of bacterial efflux pumps is that they are under very tight regulatory control and thus considered “silent ” in wild-type strains because inducing conditions are usually un-known. For this reason, such endeavors are restricted to clin-ical or laboratory-induced mutants overexpressing these pumps, but such mutants are scarce in many bacterial species, espe-cially those whose use is restricted or those that are difficult to cultivate and genetically modify. In this study, we describe a
Keyphrases
surrogate pseudomonas aeruginosa strain single-copy efflux pump gene regulated expression uncharacterized bacterial efflux pump multidrug resistance pump biosafe pseudomonas aeruginosa host burkholderia pseudomallei bpeef-oprc operon wild-type strain recombinant mini-tn7 expression vector bacterial pathogenicity major role bacterial antibiotic resistance bacterial efflux pump tight regulatory control trimethoprim efflux pump applicable method many bacterial specie laboratory-induced mutant major handicap
