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Review Structural basis for sensory rhodopsin function (2002)
BibTeX
@MISC{Pebay-peyroulaa02reviewstructural,
author = {Eva Pebay-peyroulaa and Antoine Royanta and Ehud M. L and Javier Navarroc},
title = {Review Structural basis for sensory rhodopsin function},
year = {2002}
}
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Abstract
The crystal structure of sensory rhodopsin II from Natronobacterium pharaonis was recently solved at 2.1 A ̊ resolution from lipidic cubic phase-grown crystals. A critical analysis of previous structure–function studies is possible within the framework of the high-resolution structure of this photoreceptor. Based on the structure, a molecular understanding emerges of the efficiency and selectivity of the photoisomerization reaction, of the interaction of the sensory receptor and its cognate transducer protein HtrII, and of the mechanism of spectral tuning in photoreceptors. The architecture of the retinal binding pocket is compact, representing a major determinant for the selective binding of the chromophore, all-trans retinal to the apoprotein, opsin. Several chromophore–protein interactions revealed by the structure were not predicted by previous mutagenesis and spectroscopic analyses. The structure suggests likely mechanisms by which photoisomerization triggers the activation of sensory rhodopsin II, and highlights the possibility of a unified mechanism of signaling mediated by sensory receptors, including visual rhodopsins. Future investigations using time-resolved crystallography, structural dynamics, and computational studies will provide the basis to unveil the molecular mechanisms of sensory receptors-mediated transmembrane signaling.
Keyphrases
sensory rhodopsin function review structural basis sensory rhodopsin ii sensory receptor cognate transducer protein htrii crystal structure critical analysis computational study selective binding molecular mechanism unified mechanism lipidic cubic phase-grown crystal future investigation previous structure function study molecular understanding emerges previous mutagenesis natronobacterium pharaonis likely mechanism several chromophore protein interaction spectral tuning all-trans retinal visual rhodopsin retinal binding pocket spectroscopic analysis high-resolution structure time-resolved crystallography major determinant photoisomerization reaction structural dynamic sensory receptors-mediated transmembrane signaling
