DMCA
Universality and Diversity of the Protein Folding Scenarios: A Comprehensive Analysis With the Aid of a Lattice Model (1996)
| Citations: | 16 - 4 self |
BibTeX
@MISC{Mirny96universalityand,
author = {Leonid A. Mirny and Victor Abkevich and Eugene I. Shakhnovich},
title = {Universality and Diversity of the Protein Folding Scenarios: A Comprehensive Analysis With the Aid of a Lattice Model},
year = {1996}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
this paper, we have presented a comprehensive analysis of the role of intermediates for folding of lattice model proteins. In previous instances, lattice models have been successful in identifying such key elements of the protein folding mechanism as a specific nucleus [10,12,53]. The important question remaining is to what extent are present results on the role of intermediates applicable to real proteins? In other words, how do the simulation results compare with experimental data? Experimentally, both scenarios described in this work (and summarized in Fig. 13) were observed for different proteins. Important examples of folding proceeding via intermediates are myoglobin [16], hen egg white lysozyme (HEWL) [18,58], barnase [17], cytochrome c at pH 7 [49], [59], and RNase A [50,51]. On the other hand, there are a growing number of examples where folding of a protein has been shown to follow a simple two-state scenario both in thermodynamics and in kinetics: chymotrypsin inhibitor 2 [19], ubiquitin at 8°C [22], cytochrome c at pH 5 [25], Ig-binding domain of staphylococcal protein G [20], SH3 [21], E. coli cold-shock protein [23], and acylcoenzyme A binding protein [24]. Moreover, the same protein can exhibit, at different temperatures, either scenario [22,53], or a single mutation can cause a switch between folding mechanisms (e.g. mutation I96®A in barnase, see [17]). This is in accord with our findings for the lattice model in which we have shown that the mechanism is not determined by the native structure but by features of the sequence, as comparison between
Keyphrases
lattice model comprehensive analysis protein folding scenario different protein single mutation ig-binding domain cold-shock protein simple two-state scenario native structure key element hen egg white lysozyme staphylococcal protein important question present result previous instance binding protein simulation result important example chymotrypsin inhibitor different temperature lattice model protein experimental data specific nucleus real protein
