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A new and rapid colorimetric determination of acetylcholinesterase activity
| Venue: | Biochem Pharmacol |
| Citations: | 450 - 0 self |
BibTeX
@ARTICLE{Ellman_anew,
author = {George L. Ellman and K. Diane Courtney and Valentino Andres and Robert M. Featherstone},
title = {A new and rapid colorimetric determination of acetylcholinesterase activity},
journal = {Biochem Pharmacol},
year = {},
pages = {88--95}
}
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Abstract
Abstract-A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described. The enzyme activity is measured by following the increase of yellow color produced from thiocholine when it reacts with dithiobisnitrobenzoate ion. It is based on coupling of these reactions: (enzyme) acetylthiocholine-----+ thiocholine + acetate thiocholine + dithiobisnitrobenzoate-+ yellow color The latter reaction is rapid and the assay is sensitive (i.e. a 10 pl sample of blood is adequate). The use of a recorder has been most helpful, but is not essential. The method has been used to study the enzyme in human erythrocytes and homogenates of rat brain, kidney, lungs, liver and muscle tissue. Kinetic constants determined by this system for erythrocyte cholinesterase are presented. The data obtained with acetyl-thiocholine as substrate are similar to those with acetylcholine.
Keyphrases
acetylcholinesterase activity rapid colorimetric determination latter reaction muscle tissue kinetic constant abstract-a photometric method cell suspension yellow color pl sample enzyme activity tissue extract erythrocyte cholinesterase rat brain dithiobisnitrobenzoate ion human erythrocyte
