DMCA
CELL RESPONSE TO RGD DENSITY IN CROSSLINKED ARTIFICIAL EXTRACELLULAR MATRIX PROTEIN FILMS
BibTeX
@MISC{_cellresponse,
author = {},
title = {CELL RESPONSE TO RGD DENSITY IN CROSSLINKED ARTIFICIAL EXTRACELLULAR MATRIX PROTEIN FILMS},
year = {}
}
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Abstract
This study examines the ability of crosslinked artificial extracellular matrix (aECM) protein films to alter cell adhesion, spreading, and migration. The aECM proteins described herein were originally designed for application in small-diameter grafts and are composed of elastin-like structural repeats and fibronectin cell-binding domains. aECM-RGD contains the RGD sequence from fibronectin, and aECM-RDG contains a scrambled cell-binding domain. Covalent attachment of poly(ethylene glycol) (PEG) to protein substrates reduced nonspecific cell adhesion to aECM-RDG-PEG but did not preclude sequence-specific adhesion of endothelial cells to aECM-RGD-PEG. Variation in ligand density was accomplished easily by mixing aECM-RGD-PEG and aECM-RDG-PEG. Increasing the density of RGD in the film resulted in more robust cell adhesion and spreading but did not have a significant effect on migration speeds. Cellbinding domain density in aECM proteins can thus be used to modulate cell adhesion and spreading and will serve as an important tool as these materials are further developed for use in tissue engineering.
Keyphrases
cell adhesion aecm protein endothelial cell domain density crosslinked artificial extracellular matrix ethylene glycol significant effect covalent attachment rgd sequence sequence-specific adhesion tissue engineering aecm-rdg contains protein film small-diameter graft important tool fibronectin cell-binding domain scrambled cell-binding domain ligand density migration speed robust cell adhesion elastin-like structural repeat nonspecific cell adhesion
