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Construction of genetic linkage map in man using restriction fragment length polymorphisom
| Venue: | Am. J. Hum Genet |
| Citations: | 455 - 1 self |
BibTeX
@ARTICLE{Botstein_constructionof,
author = {David Botstein and Raymond L. White and Mark Skolnick and Ronald W. Davis},
title = {Construction of genetic linkage map in man using restriction fragment length polymorphisom},
journal = {Am. J. Hum Genet},
year = {},
pages = {314331}
}
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Abstract
We describe a new basis for the construction of a genetic linkage map of the human genome. The basic principle of the mapping scheme is to develop, by recombinant DNA techniques, random single-copy DNA probes capable of detecting DNA sequence polymorphisms, when hybridized to restriction digests of an individual's DNA. Each of these probes will define a locus. Loci can be expanded or contracted to include more or less polymorphism by further application of recombinant DNA technology. Suitably polymorphic loci can be tested for linkage relationships in human pedigrees by established methods; and loci can be arranged into linkage groups to form a true genetic map of "DNA marker loci. " Pedigrees in which inherited traits are known to be segregating can then be analyzed, making possible the mapping of the gene(s) responsible for the trait with respect to the DNA marker loci, without requiring direct access to a specified gene's DNA. For inherited diseases mapped in this way, linked DNA marker loci can be used predictively for genetic counseling.
Keyphrases
genetic linkage map restriction fragment length polymorphisom dna marker locus genetic counseling recombinant dna technique human genome polymorphic locus basic principle direct access mapping scheme inherited trait linkage relationship dna sequence polymorphism linkage group human pedigree new basis inherited disease true genetic map random single-copy dna probe recombinant dna technology
