DMCA
An Application of the Molecular Replacement Technique in Direct Space to a Known Protein Structure (1975)
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BibTeX
@MISC{Argos75anapplication,
author = {Patrick Argos and Geoffrey C. Ford and Michael G. Rossmann},
title = {An Application of the Molecular Replacement Technique in Direct Space to a Known Protein Structure},
year = {1975}
}
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Abstract
The molecular replacement technique in real space (successive cycles of electron density calculations, averaging among non-crystallographically equivalent subunits, and structure factor calculations based upon the improved averaged density) was applied to glyceraldehyde-3-phosphate dehydrogenase. It was shown possible (i) to extend the phases from a known 6.0/ ~ single isomorphous replacement set to at least 4.9. ~ resolution; (ii) to determine a reasonable structure at low resolution given the molecular envelope and non-crystallographic symmetry; and (iii) to use the resulting low-resolution phases in the determination of heavy-atom sites. The application of such procedures to the determination of virus structures and heavy-atom derivative sites is discussed.
Keyphrases
molecular replacement technique direct space known protein structure heavy-atom site real space glyceraldehyde-3-phosphate dehydrogenase non-crystallographic symmetry single isomorphous replacement successive cycle non-crystallographically equivalent subunit structure factor calculation low resolution reasonable structure molecular envelope virus structure improved averaged density heavy-atom derivative site low-resolution phase electron density calculation
