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Ectopic Overexpression of The Transcription Factor OsGLK1 Induces Chloroplast Development in Non-Green
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BibTeX
@MISC{Cells_ectopicoverexpression,
author = {Rice Cells and Hidemitsu Nakamura and Masayuki Muramatsu and Makoto Hakata and Osamu Ueno and Yoshiaki Nagamura and Hirohiko Hirochika and Makoto Takano and Hiroaki Ichikawa},
title = {Ectopic Overexpression of The Transcription Factor OsGLK1 Induces Chloroplast Development in Non-Green},
year = {}
}
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Abstract
For systematic and genome-wide analyses of rice gene functions, we took advantage of the full-length cDNA overexpresser (FOX) gene-hunting system and generated> 12 000 independent FOX-rice lines from> 25 000 rice calli treated with the rice-FOX Agrobacterium library. We found two FOX-rice lines generating green calli on a callusinducing medium containing 2,4-D, on which wild-type rice calli became ivory yellow. In both lines, OsGLK1 cDNA encoding a GARP transcription factor was ectopically overexpressed. Using rice expression-microarray and northern blot analyses, we found that a large number of nucleus-encoded genes involved in chloroplast functions were highly expressed and transcripts of plastid-encoded genes, psaA, psbA and rbcL, increased in the OsGLK1-FOX calli. Transmission electron microscopy showed the existence of differentiated chloroplasts with grana stacks
Keyphrases
ectopic overexpression chloroplast function rice-fox agrobacterium library independent fox-rice line wild-type rice callus green callus plastid-encoded gene rice expression-microarray rice gene function ivory yellow genome-wide analysis transmission electron microscopy grana stack full-length cdna overexpresser osglk1-fox callus differentiated chloroplast nucleus-encoded gene northern blot analysis garp transcription factor osglk1 cdna large number fox-rice line gene-hunting system rice callus
