DMCA
Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients
| Venue: | PLoS ONE |
| Citations: | 7 - 0 self |
BibTeX
@ARTICLE{Lehmann_plasmacytoiddendritic,
author = {Clara Lehmann and Mark Lafferty and Alfredo Garzino-demo and Norma Jung and Pia Hartmann and Jeffrey S. Wolf and Jan Van Lunzen and Fabio Romerio},
title = {Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients},
journal = {PLoS ONE},
year = {},
pages = {11110}
}
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Abstract
Circulating plasmacytoid dendritic cells (pDC) decline during HIV-1 infection, but at the same time they express markedly higher levels of interferon alpha (IFNa), which is associated with HIV-1 disease progression. Here we show an accumulation of pDC in lymph nodes (LN) of treatment-naïve HIV-1 patients. This phenomenon was associated with elevated expression of the LN homing marker, CCR7, on pDC in peripheral blood of HIV-1 patients, which conferred increased migratory capacity in response to CCR7 ligands in ex vivo functional assays. LN-homed pDC of HIV-1 patients presented higher CD40 and lower BDCA2 levels, but unchanged CD83 and CD86 expression. In addition, these cells expressed markedly higher amounts of IFNa compared to uninfected individuals, and were undergoing faster rates of cell death. These results demonstrate for the first time that in asymptomatic, untreated HIV-1 patients circulating pDC up-regulate CCR7 expression, accumulate in lymph nodes, and express high amounts of IFNa before undergoing cell death. Since IFNa inhibits cell proliferation and modulates immune responses, chronically high levels of this cytokine in LN of HIV-1 patients may impair differentiation and immune
Keyphrases
hiv-1 patient lymph node secrete interferon alpha plasmacytoid dendritic cell accumulate cell death interferon alpha unchanged cd83 hiv-1 disease progression undergoing faster rate uninfected individual high amount cd86 expression ifna inhibits elevated expression plasmacytoid dendritic cell pdc up-regulate ccr7 expression hiv-1 infection bdca2 level high level treatment-na ve hiv-1 patient ccr7 ligand ln-homed pdc peripheral blood functional assay migratory capacity modulates immune response ln homing marker first time untreated hiv-1 patient
