DMCA
Mouse tissues express multiple splice variants of prominin-1
Cached
Download Links
| Venue: | PLoS ONE 2010; 5:e12325. [PMID: 20808829] © 2011 Molecular Vision |
| Citations: | 2 - 0 self |
BibTeX
@INPROCEEDINGS{Kemper_mousetissues,
author = {Kristel Kemper and Marc J. P. M. Tol and Jan Paul Medema},
title = {Mouse tissues express multiple splice variants of prominin-1},
booktitle = {PLoS ONE 2010; 5:e12325. [PMID: 20808829] © 2011 Molecular Vision},
year = {}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
Prominin-1, a heavily glycosylated pentaspan membrane protein, is mainly known for its function as a marker for (cancer) stem cells, although it can also be detected on differentiated cells. Mouse prominin-1 expression is heavily regulated by splicing in eight different variants. The function or the expression pattern of prominin-1 and its splice variants (SVs) is thus far unknown. In this study, we analyzed the expression of the prominin-1 splice variants on mRNA level in several mouse tissues and found a broad tissue expression of the majority of SVs, but a specific set of SVs had a much more restricted expression profile. For instance, the testis expressed only SV3 and SV7. Moreover, SV8 was solely detected in the eye. Intriguingly, prominin-1 knockout mice do not suffer from gross abnormalities, but do show signs of blindness, which suggest that SV8 has a specific function in this tissue. In addition, databases searches for putative promoter regions in the mouse prominin-1 gene revealed three potential promoter regions that could be linked to specific SVs. Interestingly, for both SV7 and SV8, a specific potential promoter region could be identified. To conclude, the majority of mouse prominin-1 splice variants are widely expressed in mouse tissues. However, specific expression of a few variants, likely driven by specific
Keyphrases
express multiple splice variant broad tissue expression specific expression specific set restricted expression profile specific svs several mouse tissue gross abnormality mouse tissue pentaspan membrane protein potential promoter region mouse prominin-1 splice variant stem cell prominin-1 knockout mouse prominin-1 splice variant splice variant mouse prominin-1 expression specific potential promoter region expression pattern specific function differentiated cell different variant mouse prominin-1 gene putative promoter region mrna level
