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Inhibition of miR-29 by TGF-beta-Smad3 Signaling through Dual Mechanisms Promotes Transdifferentiation of Mouse Myoblasts into Myofibroblasts (2012)
| Citations: | 6 - 0 self |
BibTeX
@MISC{Zhou12inhibitionof,
author = {Liang Zhou and Lijun Wang and Leina Lu and Peiyong Jiang and Hao Sun and Huating Wang},
title = {Inhibition of miR-29 by TGF-beta-Smad3 Signaling through Dual Mechanisms Promotes Transdifferentiation of Mouse Myoblasts into Myofibroblasts},
year = {2012}
}
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Abstract
MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression in post-transcriptional fashion, and emerging studies support their importance in regulating many biological processes, including myogenic differentiation and muscle development. miR-29 is a promoting factor during myogenesis but its full spectrum of impact on muscle cells has yet to be explored. Here we describe an analysis of miR-29 affected transcriptome in C2C12 muscle cells using a high throughput RNA-sequencing platform. The results reveal that miR-29 not only functions to promote myogenic differentiation but also suppresses the transdifferentiation of myoblasts into myofibroblasts. miR-29 inhibits the fibrogenic differentiation through down-regulating both extracellular matrix genes and cell adhesion genes. We further demonstrate that miR-29 is under negative regulation by TGF-beta (TGF-b)–Smad3 signaling via dual mechanisms of both inhibiting MyoD binding and enhancing Yin Yang 1 (YY1)-recruited Polycomb association. Together, these results identify miR-29 as a pleiotropic
Keyphrases
dual mechanism promotes transdifferentiation tgf-beta-smad3 signaling mouse myoblasts myogenic differentiation c2c12 muscle cell high throughput rna-sequencing platform muscle cell fibrogenic differentiation many biological process polycomb association non-coding rna dual mechanism cell adhesion gene muscle development post-transcriptional fashion negative regulation full spectrum mir-29 affected transcriptome extracellular matrix gene gene expression yin yang myod binding
