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Toxicity of cerium oxide nanoparticles in human lung cancer cells (2006)
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| Venue: | Int J Toxicol |
| Citations: | 21 - 2 self |
BibTeX
@ARTICLE{Lin06toxicityof,
author = {Weisheng Lin and Yue-wern Huang and Xiao-dong Zhou and Yinfa Ma},
title = {Toxicity of cerium oxide nanoparticles in human lung cancer cells},
journal = {Int J Toxicol},
year = {2006},
pages = {451--457}
}
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Abstract
With the fast development of nanotechnology, the nanomaterials start to cause people’s attention for potential toxic effect. In this paper, the cytotoxicity and oxidative stress caused by 20-nm cerium oxide (CeO2) nanoparticles in cultured human lung cancer cells was investigated. The sulforhodamine B method was employed to assess cell viability after exposure to 3.5, 10.5, and 23.3 µg/ml of CeO2 nanoparticles for 24, 48, and 72 h. Cell viability decreased significantly as a function of nanoparticle dose and exposure time. Indicators of oxidative stress and cytotoxicity, including total reactive oxygen species, glutathione, malondialdehyde, α-tocopherol, and lactate dehydrogenase, were quantitatively assessed. It is concluded from the results that free radicals generated by exposure to 3.5 to 23.3 µg/ml CeO2 nanoparticles produce significant oxidative stress in the cells, as reflected by reduced glutathione and α-tocopherol levels; the toxic effects of CeO2 nanoparticles are dose dependent and time dependent; elevated oxidative stress increases the production of malondialdehyde and lactate dehydrogenase, which are indicators of lipid peroxidation and cell membrane damage, respectively.
Keyphrases
cerium oxide nanoparticles human lung cancer cell oxidative stress ceo2 nanoparticles lactate dehydrogenase cell viability people attention time dependent lipid peroxidation exposure time fast development reduced glutathione nanoparticle dose cell membrane damage tocopherol level toxic effect 20-nm cerium oxide total reactive oxygen specie sulforhodamine method free radical potential toxic effect significant oxidative stress ml ceo2 cultured human lung cancer cell
