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Nuclear encoding of a plastid σ factor in rice and its tissue- and light-dependent expression (1997)
| Citations: | 3 - 3 self |
BibTeX
@MISC{Tozawa97nuclearencoding,
author = {Yuzuru Tozawa and Kan Tanaka and Hideo Takahashi and Kyo Wakasa},
title = {Nuclear encoding of a plastid σ factor in rice and its tissue- and light-dependent expression},
year = {1997}
}
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Abstract
DDBJ/EMBL/GenBank accession no. AB005290 A full-length cDNA encoding a putative σ factor for a plastid RNA polymerase was isolated from the higher plant Oryza sativa. The nucleotide sequence of the corresponding nuclear gene, named Os-sigA (O.sativa sigma A), predicts a polypeptide of 519 amino acids that contains a putative plastid-targeting sequence in its N-terminal region. The predicted mature protein shows extensive sequence homology to bacterial σ factors, encompassing the conserved regions 1.2, 2.1, 2.2, 2.3, 2.4, 3, 4.1 and 4.2 implicated in binding to –10 promoter elements, promoter melting and interaction with the core RNA polymerase enzyme. RNA blot analysis revealed that the abundance of Os-sigA transcripts was markedly greater in green shoots than in roots or in dark-grown etiolated shoots of rice seedlings. Furthermore, exposure of dark-grown etiolated seedlings to light resulted in a rapid increase in the amount of Os-sigA mRNA in the shoot. These observations suggest that regulation of expression of the nuclear gene for this putative plastid RNA polymerase σ factor by light contributes to lightdependent transcriptional regulation of plastid genes.
Keyphrases
plastid factor light-dependent expression nuclear encoding amino acid rapid increase nuclear gene green shoot plastid gene putative factor nucleotide sequence rna blot analysis corresponding nuclear gene full-length cdna extensive sequence homology putative plastid rna polymerase factor rice seedling light contributes ddbj embl genbank accession promoter element plastid rna polymerase dark-grown etiolated shoot transcriptional regulation predicted mature protein bacterial factor promoter melting conserved region core rna polymerase enzyme plant oryza sativa n-terminal region putative plastid-targeting sequence dark-grown etiolated seedling os-siga mrna os-siga transcript
