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Summaries of Affymetrix GeneChip probe level data (2003)
| Venue: | Nucleic Acids Res |
| Citations: | 463 - 21 self |
BibTeX
@ARTICLE{Irizarry03summariesof,
author = {Rafael A. Irizarry and Benjamin M. Bolstad and Francois Collin and Leslie M. Cope and Bridget Hobbs and Terence P. Speed},
title = {Summaries of Affymetrix GeneChip probe level data},
journal = {Nucleic Acids Res},
year = {2003},
pages = {15}
}
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Abstract
High density oligonucleotide array technology is widely used in many areas of biomedical research for quantitative and highly parallel measurements of gene expression. Affymetrix GeneChip arrays are the most popular. In this technology each gene is typically represented by a set of 11±20 pairs of probes. In order to obtain expression measures it is necessary to summarize the probe level data. Using two extensive spike-in studies and a dilution study, we developed a set of tools for assessing the effectiveness of expression measures. We found that the performance of the current version of the default expression measure provided by Affymetrix Microarray Suite can be signi®cantly improved by the use of probe level summaries derived from empirically motivated statistical models. In particular, improvements in the ability to detect differentially expressed genes are demonstrated.
Keyphrases
affymetrix genechip level data expression measure gene expression probe level data many area probe level summary extensive spike-in study array technology statistical model default expression measure biomedical research current version affymetrix microarray suite high density affymetrix genechip array dilution study parallel measurement
