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doi:10.1093/ecam/nen068 Original Article Carthamus tinctorius Enhances the Antitumor Activity of (2008)
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@MISC{Cell08doi:10.1093/ecam/nen068original,
author = {Dendritic Cell and Vaccines Polarization and Th Cytokines and Increase Cytotoxic and T Lymphocytes},
title = {doi:10.1093/ecam/nen068 Original Article Carthamus tinctorius Enhances the Antitumor Activity of},
year = {2008}
}
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Abstract
Copyright © 2011 Jia-Ming Chang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Carthamus tinctorius (CT), also named safflower, is a traditional Chinese medicine widely used to improve blood circulation. CT also has been studied for its antitumor activity in certain cancers. To investigate the effects of CT on the dendritic cell (DC)-based vaccine in cancer treatment, cytokine secretion of mouse splenic T lymphocytes and the maturation of DCs in response to CT were analyzed. To assess the antitumor activity of CT extract on mouse CD117 + (c-kit)-derived DCs pulsed with JC mammal tumor antigens, the JC tumor was challenged by the CT-treated DC vaccine in vivo. CT stimulated IFN-γ and IL-10 secretion of splenic T lymphocytes and enhanced the maturation of DCs by enhancing immunological molecule expression. When DC vaccine was pulsed with tumor antigens along with CT extract, the levels of TNF-α and IL-1β were dramatically increased with a dosedependent response and more immunologic and co-stimulatory molecules were expressed on the DC surface. In addition, CTtreated tumor lysate-pulsed DC vaccine reduced the tumor weight in tumor-bearing mice by 15.3 % more than tumor lysate-pulsed DC vaccine without CT treatment. CT polarized cytokine secretion toward the Th1 pathway and also increased the population of cytotoxic T lymphocytes ex vivo. In conclusion, CT activates DCs might promote the recognition of antigens and facilitate antigen presentation to Th1 immune responses. 1.
Keyphrases
antitumor activity original article carthamus tinctorius cytokine secretion ct extract original work jc mammal tumor antigen dendritic cell traditional chinese medicine splenic lymphocyte tumor weight antigen presentation ct-treated dc vaccine dc vaccine ct treatment creative common attribution license dc surface co-stimulatory molecule mouse cd117 th1 immune response jia-ming chang tumor lysate-pulsed dc vaccine certain cancer unrestricted use jc tumor dosedependent response th1 pathway ex vivo immunological molecule expression tumor antigen cancer treatment il-10 secretion tumor-bearing mouse blood circulation open access article cttreated tumor lysate-pulsed dc vaccine mouse splenic lymphocyte carthamus tinctorius
