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Diversity of Raft-Like Domains in Late Endosomes
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@MISC{Sobo_diversityof,
author = {Komla Sobo and Julien Chevallier and Robert G. Parton and Jean Gruenberg and F. Gisou Van Der Goot},
title = {Diversity of Raft-Like Domains in Late Endosomes},
year = {}
}
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Abstract
Background. Late endosomes, the last sorting station in the endocytic pathway before lysosomes, are pleiomorphic organelles composed of tubular elements as well as vesicular regions with a characteristic multivesicular appearance, which play a crucial role in intracellular trafficking. Here, we have investigated whether, in addition to these morphologically distinguishable regions, late endosomal membranes are additionally sub-compartmentalized into membrane microdomains. Methodology/Principal Findings. Using sub-organellar fractionation techniques, both with and without detergents, combined with electron microscopy, we found that both the limiting membrane of the organel and the intraluminal vesicles contain raft-type membrane domains. Interestingly, these differentially localized domains vary in protein composition and physico-chemical properties. Conclusions/Significance. In addition to the multivesicular organization, we find that late endosomes contain cholesterol rich microdomains both on their limiting membrane and their intraluminal vesicles that differ in composition and properties. Implications of these findings for late endosomal functions are discussed.
Keyphrases
late endosomes raft-like domain intraluminal vesicle intracellular trafficking membrane microdomains vesicular region raft-type membrane domain electron microscopy pleiomorphic organelle limiting membrane multivesicular organization distinguishable region methodology principal finding last sorting station crucial role endocytic pathway characteristic multivesicular appearance tubular element conclusion significance late endosomal membrane protein composition localized domain late endosomal function sub-organellar fractionation technique physico-chemical property
