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Crystalline tubes of myosin subfragment-2 showing the coiled-coil and molecular interaction geometry (1987)
| Venue: | J. Celt Biol |
| Citations: | 5 - 0 self |
BibTeX
@ARTICLE{Quinlan87crystallinetubes,
author = {Roy A. Quinlan and Murray Stewart},
title = {Crystalline tubes of myosin subfragment-2 showing the coiled-coil and molecular interaction geometry},
journal = {J. Celt Biol},
year = {1987}
}
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Abstract
Abstract. We have produced crystalline tubes of chicken breast myosin long subfragment-2 that show order to resolutions better than 2 nm. The tubes were formed from a thin sheet in which the myosin long subfragment-2 molecules were arranged on an approximately rectangular crystalline lattice with a = 14.1 + 0.2 nm and b-- 3.9 + 0.1 nm in projection. Shadowing indicated that the tube wall was ~7 nm thick and that the sheets from which it was formed followed a right-handed helix. Superposition of the lattices from the top and bottom of the tube produced a moire pattern in negatively stained material, but images of single sheets were easily obtained by computer image processing. Although several molecules were superimposed perpendicular to the plane of the sheet, the
