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Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae (1998)
| Citations: | 10 - 2 self |
BibTeX
@MISC{Segal98clb5-associatedkinase,
author = {Marisa Segal and Duncan J. Clarke and Steven I. Reed},
title = {Clb5-associated kinase activity is required early in the spindle pathway for correct preanaphase nuclear positioning in Saccharomyces cerevisiae},
year = {1998}
}
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Abstract
Abstract. In Saccharomyces cerevisiae, a single cyclindependent kinase, Cdc28, regulates both G1/S and G2/M phase transitions by associating with stage-specific cyclins. During progression through S phase and G2/M, Cdc28 is activated by the B-type cyclins Clb1–6. Because of functional redundancy, specific roles for individual Clbs have been difficult to assign. To help genetically define such roles, strains carrying a cdc28 ts allele, combined with single CLB deletions were studied. We assumed that by limiting the activity of the kinase, these strains would be rendered more sensitive to loss of individual Clbs. By this approach, a novel phenotype associated with CLB5 mutation was observed. Homozygous cdc28-4 ts clb5 diploids were inviable at room temperature. Cells
Keyphrases
saccharomyces cerevisiae spindle pathway correct preanaphase nuclear positioning clb5-associated kinase activity individual clbs g2 phase transition single clb deletion clb5 mutation homozygous cdc28-4 t clb5 diploid cdc28 t allele single cyclindependent kinase stage-specific cyclins room temperature specific role functional redundancy b-type cyclins clb1 novel phenotype
