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Substructure of Inner Dynein Arms, Radial Spokes, and the Central Pair/Projection Complex of Cilia and Flagella
| Citations: | 45 - 2 self |
BibTeX
@MISC{Goodenough_substructureof,
author = {Ursula W. Goodenough and John E. Heuser},
title = {Substructure of Inner Dynein Arms, Radial Spokes, and the Central Pair/Projection Complex of Cilia and Flagella},
year = {}
}
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Abstract
ABSTRACT The substructure of the components of the axoneme interior--the inner dynein arms, the radial spokes, and the central pair/projection complex--was analyzed for Chlamy-domonas, Tetrahymena, Strongelocentrotus, and Mnemiopsis using the quick-freeze, deep-etch technique. The inner arms are shown to resemble the outer arms in overall molecular organization, but they are disposed differently on the microtubule and have two distinct morphologies--dyads with two heads and triads with three. The dyads associate with spokes $3 and $2; the triads associate with $1. The spokes form a three-start right-handed helix with a 288-nm rise; the central pair makes a shallow left-handed twist. The spoke heads are shown to be made up of four major subunits; two bind to the spoke shaft and two bind to a pair of central-sheath projections. Most motile cilia contain outer and inner dynein arms, radial spokes, and a central pair/projection complex. Mutations affecting the assembly of any of these structures can result in immotility (1); therefore, an understanding of ciliary motility must ultimately include a description of the role played by
Keyphrases
radial spoke inner dynein arm central pair projection complex distinct morphology central-sheath projection inner arm three-start right-handed helix spoke head major subunit spoke shaft overall molecular organization motile cilium contain outer shallow left-handed twist spoke $3 288-nm rise outer arm deep-etch technique ciliary motility central pair
