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Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): I. CIP Treatment of Samples and Tailing Reaction with Terminal Transferase
by
Chih Long Liu
,
Bradley E. Bernstein
,
Stuart L. Schreiber
BibTeX
@MISC{Liu_wholegenome,
author = {Chih Long Liu and Bradley E. Bernstein and Stuart L. Schreiber},
title = {Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): I. CIP Treatment of Samples and Tailing Reaction with Terminal Transferase},
year = {}
}
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Abstract
T7-based linear amplification of DNA (TLAD) uses a linear amplification approach based on in vitro transcription (IVT) of template DNA by RNA polymerase from T7 phage. TLAD was designed for use with the ChIP-chip method (whereby DNA recovered from chromatin immunoprecipitation [ChIP] of cell lysate is used for subsequent analysis on DNA microarrays) and requires nanogram quantities of
