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ORIGINAL ARTICLES Methyl-b-Cyclodextrins Preferentially Remove Cholesterol from the Liquid Disordered Phase in Giant Unilamellar Vesicles
BibTeX
@MISC{Sanchez_originalarticles,
author = {S. A. Sanchez and E. Gratton and S. A. Sanchez and G. Gunther and M. A. Tricerri},
title = {ORIGINAL ARTICLES Methyl-b-Cyclodextrins Preferentially Remove Cholesterol from the Liquid Disordered Phase in Giant Unilamellar Vesicles},
year = {}
}
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Abstract
Ó The Author(s) 2011. This article is published with open access at Springerlink.com Abstract Methyl-b-cyclodextrins (MbCDs) are molecules that are extensively used to remove and to load cholesterol (Chol) from artificial and natural membranes; however, the mechanism of Chol extraction by MbCD from pure lipids or from complex mixtures is not fully understood. One of the outstanding questions in this field is the capability of MbCD to remove Chol from lipid domains having different packing. Here, we investigated the specificity of MbCD to remove Chol from coexisting macrodomains with different lipid packing. We used giant unilamellar vesicles (GUVs) made of 1,2-dioleoylphosphatidylcholine:1,2-dipalmitoylphatidylcholine:free cholesterol, 1:1:1 molar ratio at 27°C. Under these conditions, individual GUVs present Chol distributed into lo and ld phases. The two phases can be distinguished and visualized using Laurdan generalized polarization and two-photon excitation fluorescence microscopy. Our data indicate that MbCD removes Chol preferentially from the more
Keyphrases
giant unilamellar vesicle liquid disordered phase free cholesterol chol extraction open access molar ratio laurdan generalized polarization two-photon excitation fluorescence microscopy natural membrane lipid domain complex mixture pure lipid different lipid packing different packing individual guvs present chol outstanding question ld phase com abstract methyl-b-cyclodextrins
