DMCA
modulate macrophage immune responses (2006)
BibTeX
@MISC{S06modulatemacrophage,
author = {Neuropeptide S and G Protein-coupled Receptor and Ville Pulkkinen and Marja-leena Majuri and Guoying Wang and Yasushi Obase and Johanna Vendelin and Henrik Wolff and Lauri A. Laitinen and Tari Haahtela and Tarja Laitinen and Harri Alenius and Juha Kere and Marko Rehn},
title = {modulate macrophage immune responses},
year = {2006}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
G protein-coupled receptor 154 (GPR154) is a recently discovered asthma susceptibility gene upregulated in the airways of asthma patients. We previously observed increased pulmonary mRNA expression of the murine ortholog Gpr154 in a mouse model of ovalbumin (OVA)-induced inflammation. However, the expression profile of GPR154 in leukocytes and the cellular functions of the receptor and its endogenous agonist neuropeptide S (NPS) have remained unidentified. Here, we characterized the mRNA expression of NPS and GPR154 by using real-time RT–PCR in fractionated human blood cells and in peripheral blood mononuclear cells (PBMCs) with monocyte or T cell activation. The expression of GPR154 in leukocytes was further confirmed by immunoblotting experiments and immunohistochemical staining of human sputum samples. Additionally, we characterized the expression of GPR154 in the lung tissue samples and in the bronchoalveolar lavage (BAL) fluid of OVA sensitized and challenged BALB/c mice. In human blood and sputum cells, monocyte/macrophages and eosinophils were identified as GPR154-positive cells. In PBMCs, monocyte activation with LPS but not T cell activation with anti-CD3/CD28 antibodies resulted in increased NPS and GPR154 expression. In the lung tissue samples and in the BAL fluid of OVA-challenged mice, GPR154 expression was upregulated in alveolar macrophages in comparison to con-trols. In the mouse macrophage RAW 264.7 cell line, NPS-stimulated Gas- and Gaq-dependent phagocytosis
Keyphrases
modulate macrophage immune response gpr154 expression lung tissue sample cell activation immunohistochemical staining cell line murine ortholog gpr154 fractionated human blood cell monocyte macrophage monocyte activation mouse macrophage raw increased np human blood nps-stimulated gas gpr154-positive cell asthma susceptibility gene increased pulmonary mrna expression bal fluid real-time rt pcr sputum cell gaq-dependent phagocytosis ova-challenged mouse human sputum sample cellular function balb mouse expression profile bronchoalveolar lavage anti-cd3 cd28 antibody protein-coupled receptor asthma patient alveolar macrophage mouse model peripheral blood mononuclear cell mrna expression
