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Nuclear localization of non-structural protein 1 and nucleocapsid protein of equine arteritis virus (2002)
| Venue: | J Gen |
| Citations: | 7 - 1 self |
BibTeX
@ARTICLE{Tijms02nuclearlocalization,
author = {Marieke A. Tijms and Yvonne Van Der Meer and Eric J. Snijder},
title = {Nuclear localization of non-structural protein 1 and nucleocapsid protein of equine arteritis virus},
journal = {J Gen},
year = {2002},
pages = {83--795}
}
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Abstract
RNA synthesis (genome replication and subgenomic mRNA transcription) directed by equine arteritis virus (EAV; family Arteriviridae, order Nidovirales) occurs on modified cytoplasmic membranes to which most viral replicase subunits localize. Re-markably, a fraction of non-structural protein 1 (nsp1), a protein essential for transcription but dis-pensable for genome replication, is present in the host cell nucleus, in particular during the earlier stagesof infection.ExpressionofGFP-tagged fusion proteins revealed that nsp1 is actively imported into the nucleus. Although the signals responsible for nsp1 transport could not be identified, our studies revealed that another EAV protein with a partially nuclear localization, the nucleocapsid (N)
Keyphrases
equine arteritis virus non-structural protein nuclear localization nucleocapsid protein genome replication nsp1 transport rna synthesis family arteriviridae stagesof infection subgenomic mrna transcription modified cytoplasmic membrane eav protein expressionofgfp-tagged fusion protein host cell viral replicase subunit order nidovirales
