DMCA
Differential nuclear scaffold/matrix attachment marks expressed genes (2009)
| Venue: | Hum. Mol. Genet |
| Citations: | 13 - 7 self |
BibTeX
@ARTICLE{Linnemann09differentialnuclear,
author = {Amelia K. Linnemann and Adrian E. Platts and Stephen A. Krawetz},
title = {Differential nuclear scaffold/matrix attachment marks expressed genes},
journal = {Hum. Mol. Genet},
year = {2009},
pages = {645--654}
}
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Abstract
It is well established that nuclear architecture plays a key role in poising regions of the genome for transcrip-tion. This may be achieved using scaffold/matrix attachment regions (S/MARs) that establish loop domains. However, the relationship between changes in the physical structure of the genome as mediated by attach-ment to the nuclear scaffold/matrix and gene expression is not clearly understood. To define the role of S/MARs in organizing our genome and to resolve the often contradictory loci-specific studies, we have sur-veyed the S/MARs in HeLa S3 cells on human chromosomes 14–18 by array comparative genomic hybridiz-ation. Comparison of LIS (lithium 3,5-diiodosalicylate) extraction to identify SARs and 2 M NaCl extraction to identify MARs revealed that approximately one-half of the sites were in common. The results presented in this study suggest that SARs 50 of a gene are associated with transcript presence whereas MARs contained within a gene are associated with silenced genes. The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution.
Keyphrases
hela s3 cell unique functional contribution nacl extraction human chromosome silenced gene scaffold matrix attachment region different extraction method loop domain key role nuclear scaffold matrix physical structure varied function contradictory loci-specific study transcript presence gene expression array comparative genomic hybridiz-ation nuclear architecture
